Application of Lactobacillus casei 01 and oligofructose-enriched inulin in ayran

Tanja Petreska Ivanovska, Zoran Zhivikj, Liljana Bogdanovska, Kristina Mladenovska, Lidija Petrushevska-Tozi


This study aimed to develop a probiotic/synbiotic ayran with a sufficient number of live bacteria Lactobacillus casei 01 (L. casei 01) during the product shelf-life. Under conditions of refrigerated storage for 15 days, probiotic survival and organic acid (lactic, acetic, propionic and butyric acid) production were evaluated. Simultaneously, pH, titratable acidity, total solids, and carbohydrate, protein, fat and mineral contents of the samples were examined. Overall results of the chemical analyses indicated the quality of the prepared samples to be comparable with the conventional ayran used as a matrix for probiotic/synbiotic supplementation. L. casei 01 survived better in ayran containing synbiotic chitosan–Ca–alginate microparticles compared to samples enriched with non-encapsulated probiotic alone or with synbiotic (L. casei 01 + oligofructose-enriched inulin), followed by increased production of lactic, acetic and propionic acid. Probiotic/synbiotic ayran may contribute to a healthy diet for consumers, while the sample enriched with microencapsulated formulation is advantageous in terms of probiotic activity.


probiotic ayran; synbiotic ayran; synbiotic microparticles; Lactobacillus casei 01; Synergy 1

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C. Ceapa, H. Wopereis, L. Rezaїki, M. Kleerebezem, J. Knol, R. Oozeer, Influence of fermented milk products, prebiotics and probiotics on microbiota composition and health, Best Pract. Res. Clin. Gastroenterol., 27(1), 139–155 (2013). DOI: 10.1016/j.bpg.2013.04.004

M. L. Marco, D. Heeney, S. Binda, C. J. Cifelli, P. D. Cotter, B. Foligné, M. Gänzle, R. kort, G. Pasin, A. Pihlanto, E. J. Smid, R. Hutkins, Health benefits of fermented foods: microbiota and beyond, Curr. Opin. Biotechnol., 44, 94–102 (2017).

DOI: 10.1016/j.copbio.2016.11.010

C. F. Balthazar, H. L. A. Silva, E. A. Esmerino, R. S. Rocha, J. Moraes, M. A. V. Carmo, L. Azevedo, I. Camps, Y. K. D. Abud, C. Sant’Anna, R. M. Franco, M. Q. Freitas, M. C. Silva, R. S. L. Raices, G. B. Escher, D. Granato, C. S. Ranadheera, F. Nazarro, A. G. Cruz, The addition of inulin and Lactobacillus casei 01 in sheep milk ice cream, Food Chem., 246, 464–472 (2018). DOI: 10.1016/j.foodchem.2017.12002

T. Petreska Ivanovska, K. Mladenovska, Z. Zhivikj, M. Jurhar Pavlova, I. Gjurovski, T. Ristoski, L. Petrushevska-Tozi, Synbiotic loaded chitosan-Ca-alginate microparticles reduces inflammation in the TNBS model of rat colitis, Int. J. Pharm., 527, 126–134 (2017). DOI: 10.1016/j.ijpharm2017.05.049

E. Vaghef-Mehrabany, B. Alipour, A. Homayouni-Rad, S. K. Sharif, M. Asghari-Jafarabadi, S. Zavvari, Probiotic supplementation improves inflammatory status in patients with rheumatoid arthritis, Nutrition, 30, 430–435 (2014). DOI:10.1016/j.nut.2013.09.007

S. Nualkaekul, D. Lenton, M. T. Cook, V. V. Khutoryanskiy, D. Charalampopoulos, Chitosan coated alginate beads for the survival of microencapsulated Lactobacillus plantarum in pomegranate juice, Carbohydr. Polym., 90(3), 1281–1287 (2012).


A. L. D. Batista, R. Silva, L. P. Cappato, C. N. Almada, R. K. A. Garcia, M. C. Silva, R. S. L. Raices, D. B. Arellano, A. S. Sant’Anna, C. A. Conte Junior, Quality parameters of probiotic yogurt added to glucose oxidase compared to commercial products through microbiological, physico-chemical and metabolic activity analyses, Food Res. Int., 77, 627–635 (2015).

DOI: 10.1016/j.foodres.2015.08.017

G. Mitropoulou, V. Nedovic, A. Goyal, Y. Kourkoutas, Immobilization technologies in probiotic food production, J. Nutr. Metab., 2013, 1–15 (2013).


M. T. Cook, G. Tzortzis, D. Charalampopoulos, V. V. Khutoryanskiy, Microencapsulation of probiotics for gastrointestinal delivery, J. Control. Release, 162, 56–67 (2012). DOI: 10.1016/j.jconrel.2012.06.003.

T. Petrović, V. Nedović, S. Dimitrijević-Branković, B. Bugarski, C. Lacroix, Protection of probiotic microorganisms by microencapsulation, Chem. Ind. Chem. Eng. Q., 13(3), 169–174 (2007).

DOI: 10.2298/CICEQ0703169P.

Turkish Food Codex: Fermented Milk Products, Regulation No: 2009/25, Ministry of Food, Agriculture and Livestock, Turkey, Ankara (2009).

F. Altay, F. Karbancioglu-Gϋler, C. Daskaya-Dikmen, D. Heperkan, A review on traditional Turkish fermented non-alcoholic beverages: microbiota, fermentation process and quality characteristics, Int. J. Food Microbiol., 167(1), 44–56 (2013).

DOI: 10.1016/j.ijfoodmicro.2013.06.016.

R. P. S. Oliveira, A. C. R. Florence, R. C. Silva, P. Perego, A. Converti, A. L. Gioeilli, M. N. Oliveira, Effect of different prebiotics on the fermentation kinetics, probiotic survival and fatty acids profiles in non-fat synbiotic fermented milk, Int. J. Food Microbiol., 128(3), 467–472 (2009). DOI: 10.1016/j.ijfoodmicro.2008.10.012.

R. P. De Souza Oliveira, P. Perego, M. N. De Oliveira, A. Converti, Effect of inulin as prebiotic and synbiotic interactions between probiotics to improve fermented milk firmness, J. Food Eng., 107(1), 36–40 (2011).

DOI: 10.1016/j.jfoodeng.2011.06.005.

A. Köksoy, M. Kiliç, Effects of water and salt level on rheological properties of ayran, a Turkish yoghurt drink, Int. Dairy J., 13, 835–839 (2003).


D. Granato, G. F. Branco, A. G. Cruz, J. A. F. Faria, N. P. Shah, Probiotic dairy products as functional foods, Compr. Rev. Food Sci. Food Safety, 9, 455–470 (2010). DOI: 10.1111/j.1541-4337.2010.00120.x.

Т. Petreska Ivanovska, L. Petrushevska-Tozi, A. Grozdanov, R. Petkovska, J. Hadjieva, E. Popovski, T. Stafilov, K. Mladenovska, From optimization of synbiotic microparticles prepared by spray-drying to development of new functional carrot juice, Chem. Ind. Chem. Eng. Q., 20(4), 549–564 (2014).

DOI: 10.2298/CICEQ130218036P.

AOAC, Official Methods of Analysis Chemists, 15th ed. Association of Official Analytical Chemists – AOAC, Virginia, Arlington, USA (1990).

D. H. Kleyn, J. M. Lynch, D. M. Barbano, M. J. Bloom, M. W. Mitchell, Determination of fat in raw and processed milks by the Gerber method: collaborative study, J. AOAC Int., 84(5), 1499–1508 (2001).

M. M. Bradford, A rapid and sensitive method for the quantitation of protein using the principle of protein-dye binding, Anal. Biochem., 72(1–2), 248–254 (1976).

DOI: 10.1016/0003-2697(76)90527-3.

D. Pan, Y. Guo, Optimization of sour milk fermentation for the production of ACE-inhibitory peptides and purification of a novel peptide from the whey protein hydrolisate, Int. Dairy J., 20, 472–479 (2010).

DOI: 10.1016/j.idairyj.2010.01.007.

B. O. Al-Abdulkarim, M. S. Osman, M. A. I. El-Nadeef, Determination of chemical composition, and storage on dried fermented goat milk product (Oggtt), J. Saudi Soc. Agric. Sci., 12, 161–166 (2013).


A. Galat, J. Dufresne, J. Combrisson, A. Thépaut, L. Boumghar-Bourtchai, M. Boyer, C. Fourmestraux, Novel method based on chromogenic media for discrimination and selective enumeration of lactic acid bacteria in fermented milk products, Food Microbiol., 55, 86–94 (2016). DOI:

T. Y. Sheu, R. T. Marshall, H. Heymann, Improving survival of culture bacteria in frozen desserts by microentrapment, J. Dairy Sci. 76(7), 1902–1907 (1993). DOI:

Y. C. Wang, R. C. Yu, H. Y. Yang, C. C. Chou, Sugar and acid contents in soymilk fermented with lactic acid bacteria alone or simultaneously with bifidobacteria, Food Microbiol. 20(3), 333–338 (2003).

DOI: 10.1016/S0740-0020(02)00125-9.

J. C. Zhao, Z. A. Guo, J. H. Chang, W. J. Wang, Study on reversed-phase high performance liquid chromatography separation condition and determination method of organic acids, Se Pu 19(3), 260–263 (2001).

F. P. De Castro, T. M. Cunha, P. J. Ogliari, R. F. Teófilo, M. M. C. Ferreira, E. S. Prudêncio, Influence of different content of cheese whey and oligofructose on the properties of fermented lactic beverages: Study using response surface methodology, LWT-Food Sci. Technol., 42, 993–997 (2009). DOI:10.1016/j.lwt.2008.12.010.

F. Miremadi, F. Sherkat, L. Stojanovska, Hypocholaesterolemic effect and anti-hypertensive properties of probiotics and prebiotics: a review. J. Funct. Foods, 25, 497–510 (2016). DOI: 10.1016/j.jff.2016.06.016.

C. Koçak, Y. K. Avşar, B. Tamuçay, A comparative study on the production methods of ayran, Gıda 31(4), 225–231 (2006).

G. Z. Brinques, M. A. Z. Ayub, Effect of microencapsulation on survival of Lactobacillus plantarum in simulated gastrointestinal conditions, refrigeration, and yogurt, J. Food Eng. 103(2), 123–128 (2011).


A. García-Ceja, E. Mani-López, E. Palou, A. López-Malo, Viability during refrigerated storage in selected food products and during simulated gastrointestinal conditions of individual and combined lactobacilli encapsulated in alginate or alginate-chitosan, LWT-Food Sci. Technol., 63(1), 482–489 (2015).


W. Krasaekoopt, B. Bhandari, H. C. Deeth, Survival of probiotics encapsulated in chitosan-coated alginate beads in yoghurt from UHT- and conventionally treated milk during storage, LWT-Food Sci. Technol. 39, 177–183 (2006). DOI: 10.1016/j.lwt.2004.12.006.

P. Chaikham, Stability of probiotics encapsulated with Thai herbal extracts in fruit juices and yoghurt during refrigerated storage, Food Biosci. 12, 61–66 (2015).

DOI: 10.1016/j.fbio.2015.07.006.

M. C. E. Ribeiro, K. S. Chaves, C. Gebara, F. N. S. Infante, C. R. F. Grosso, M. L. Gigante, Effect of microencapsulation of Lactobacillus acidophilus LA-5 on physicochemical, sensory and microbiological characteristics of stirred probiotic yoghurt, Food Res. Int., 66, 424–431 (2014). DOI: 10.1016/j.foodres.2014.10.019.

J. Tan, C. McKenzie, M. Potamitis, A. N. Thornburn, C. R. Mackay, L. Macia, The role of short-chain fatty acids in health and disease, Adv. Immunol., 121, 91–119 (2014). DOI: 10.1016/B978-0-12-800100-4.00003-9.

O. Donkor, S. Nilmini, P. Stolic, T. Vasiljevic, N. P. Shah, Survival and activity of selected probiotic organisms in set-type yoghurt during cold storage, Int. Dairy J., 17(6), 657–665 (2007).




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