Physicochemical characterization and bioactive compounds of stalk from hot fruits of Capsicum annuum L.

Authors

  • Jana Simonovska Faculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Rudjer Boskovic 16, 1000 Skopje, Macedonia Faculty of Chemistry and Chemical Engineering, University in Maribor Smetanova 17, 2000 Maribor, Slovenia
  • Mojca Škerget Faculty of Chemistry and Chemical Engineering, University in Maribor Smetanova 17, 2000 Maribor
  • Željko Knez Faculty of Chemistry and Chemical Engineering, University in Maribor Smetanova 17, 2000 Maribor
  • Marija Srbinoska Scientific Tobacco Institute, University St. Kliment Ohridski – Bitola Kicevska bb, 7500 Prilep
  • Zoran Kavrakovski Faculty of Pharmacy, Ss. Cyril and Methodius University, Majka Tereza 47, 1000 Skopje
  • Anita Grozdanov Faculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Rudjer Boskovic 16, 1000 Skopje
  • Vesna Rafajlovska Faculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Rudjer Boskovic 16, 1000 Skopje

DOI:

https://doi.org/10.20450/mjcce.2016.944

Keywords:

red hot pepper stalk, physicochemical characterization, nutritive value, bioactive compounds, thermogravimetric analysis

Abstract

In stalk of red hot pepper (Capsicum annuum L.) with 0.25 mm, 0.5 mm and 1.0 mm particle sizes, the quantities of nutritive and volatile compounds, bioactive capsaicin and carotenoids were determined. Furthermore, the stalk was characterized using TGA, DSC and FTIR spectroscopy. The influence of the particle size on the content of proteins, ash and cellulose is insignificant. Compared to 0.5 and 1.0 mm, stalk with 0.25 mm particle size was darker with a deeper yellow-brown color, and richer in microelements. Among the quantified volatile compounds, 6 were esters, 2 terpenoids, and 1 acid. The highest quantity of extract rich fats was obtained from stalk with 0.25 mm particle size by using ethanol, which is more efficient for capsaicin extraction, while n-hexane is efficient for extraction of carotenoids. Stalk with 0.25 mm particle size was characterized with a higher degradation temperature and residual weight.

Author Biography

Jana Simonovska, Faculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Rudjer Boskovic 16, 1000 Skopje, Macedonia Faculty of Chemistry and Chemical Engineering, University in Maribor Smetanova 17, 2000 Maribor, Slovenia

Department of Food Technology and Biotechnology, Institute of Organic Chemistry

References

M. R. S. Campos, K. R. Gómez, Y. M. Ordoñez, D. B. Ancona, Polyphenols, ascorbic acid and carotenoids contents and antioxidant properties of habanero pepper (Capsicum chinense) fruit, Food Nutr. Sci. 4, 47–54 (2013). DOI: org/10.4236/fns.2013.48A006.

A. Perva-Uzunalić, M. Škerget, B. Weinreich, Ž. Knez, Extraction of chilli pepper (var. Byedige) with supercritical CO2: Effect of pressure and temperature on capsaicinoid and colour extraction efficiency, Food Chem. 87(1), 51−58 (2004).

DOI: 10.1016/j.foodchem.2003.10.016.

I. Guzman, P. W. Bosland, M. A. O’Connell, Heat, Color, and Flavor Compounds in Capsicum Fruit, in: The Biological Activity of Phytochemicals, Recent Advances in Phytochemistry 41, D. R. Gang (Ed), Springer Science+Business Media, Springer: New York, Dordrecht, Heidelberg, London, 2011, pp. 109–126.

C. B. Davis, C. E. Markey, M. A. Busch, K. W. Busch, Determination of capsaicinoids in habanero peppers by chemometric analysis of UV spectral data, J. Agric. Food Chem. 55, 5925–5933 (2007).

DOI: 10.1021/jf070413k.

H. Knotkova, M. Pappagallo, A. Szallasi, Capsaicin (TRPV1 agonist) therapy for pain relief: farewell or re-vival, Clin. J. Pain 24(2), 143–154 (2008).

DOI: 10.1097/AJP.0b013e318158ed9e.

Y. J. Surh, K. J. Kundu, Molecular mechanisms of chemoprevention with capsaicinoids from chili peppers, in: Vegetables, whole grains, and their derivatives in cancer prevention, M. Mutanen, A. M. Pajari (Eds), Dordrecht, Heidelberg, London, New York: Springer Science+Business Media 2011, pp. 123–142.

H. C. Reinbach, A. Smeets, T. Martinussen, P. Moller, S. Westerterp-Plantenga, Effects of capsaicin, green tea and CH-19 sweet pepper on appetite and energy intake in humans in negative and positive energy balance, Clin. Nutr. 28, 260–265 (2009).

DOI: org/10.1016/j.clnu.2009.01.010.

P. Wang, D. Liu, Z. Zhu, Transient receptor potential vanilloid type-1 channel in cardiometabolic protection, J. Korean Soc. Hypertns. 17(2), 37–47 (2001). DOI:10.5646/jksh.2011.17.2.37.

K. Chaiyasit, W. Khovidhunkit, S. Wittayalertpanya, Pharmacokinetic and the effect of capsaicin in Capsicum frutescens on decreasing plasma glucose level, J. Med. Assoc. Thai 92(1), 108–113 (2009).

C. C. Uzoh, V. Kumar, A. G. Timoney, The use of capsaicin in loin pain-haematuria syndrome, BJU Int. 103, 236–239 (2008).

T. Singh, C. Chittenden, In-vitro antifungal activity of chilli extracts in combination with Lactobacillus casei against common sapstain fungi, Int. Biodeter. Biodegr. 62(4), 364–367 (2008).

DOI: 10.1016/j.ibiod.2007.10.009.

E. J. Mendelson, K. B. Tolliver, L. K. Delucchi, J. B. Matthew, C. K. F. Wilson-Harris P. G. Galloway, P. Berger, Capsaicin, an active ingredient in pepper sprays, increases the lethality of cocaine, Forensic Toxicol. 28, 33–37 (2010). DOI: 10.1007/s11419-009-0079-9.

T. J. Dougnon, P. Kiki, T. V. Dougnon, I. Youssao, Evaluation of Capsicum frutescens powder effects on the growth performances, biochemical and hematological parameters in Hubbard broiler, J. App. Pharm. Sci. 4(10), 38–43 (2014). DOI: 10.7324/JAPS.2014.40107.

M. Del Rocío Gómez-García, N. Ochoa-Alejo, Bio-chemistry and molecular biology of carotenoid biosynthesis in chili peppers (Capsicum spp.), Int. J. Mol. Sci. 14, 19025−19053 (2013). DOI: 10.3390/ijms140919025.

M. Hernández-Ortega, A. Ortiz-Moreno, M. D. Hernández-Navarro, G. Chamorro-Cevallos, L. Dorantes-Alvarez, H. Necoechea-Mondragón, Antioxidant, anti-nociceptive, and anti-inflammatory effects of carotenoids extracted from dried pepper (Capsicum annuum L.), J. Biomed. Biotechnol. 1−10 (2012).

DOI:10.1155/2012/524019.

O. C. Perera, G. M. Yen, Functional properties of carotenoids in human health, Int. J. Food Prop. 10(2), 201−230 (2007). DOI: 10.1080/10942910601045271.

J. Simonovska, V. Rafajlovska, Z. Kavrakovski, M. Srbinoska, Nutritive and bioactive compounds in hot fruits of Capsicum annuum L. from Macedonia, Maced. J. Chem. Chem. Eng. 33(1), 97–104 (2014).

L. Chen, J. E. Hwang, K. M. Gu, J. H. Kim, B. Choi, S. K. Song, Y. Park, Y. K. Kang, Comparative study of antioxidant effects of five Korean varieties red pepper (Capsicum annuum L.) extracts from various parts including placenta, stalk, and pericarp, Food Sci. Biotechnol. 21(3), 715−721 (2012).

DOI: 10.1007/s10068-012-0093-2.

L. Chen, Y. H. Kang, Anti-inflammatory and antioxidant activities of red pepper (Capsicum annuum L.) stalk extracts: Comparison of pericarp and placenta extracts, J. Funct. Food 5(4), 1724–1731 (2013).

DOI: .org/10.1016/j.jff.2013.07.018.

L. Chen, J. E. Hwang, B. Choi, K. M. Gu, Y. Park, Y. K. Kang, Antioxidant capacities and cytostatic effect of Korean red pepper (Capsicum annuum L): A screening and in vitro study, J. Korean Soc. Applied Biol. Chem. 57, 43−52 (2014). DOI: 10.1007/s13765-013-4152-z.

W. Niu, L. Han, X. Liu, G. Huang, L. Chen, W. Xiao, Z. Yang, Twenty-two compositional characterizations and theoretical energy potentials of extensively diversified China's crop residues, Energy 100, 238−250 (2016).

DOI: org/10.1016/j.energy.2016.01.093.

T. G. Bridgeman, L. I. Darvell, J. M. Jones, P. T. Williams, R. Fahmi, A. V. Bridgwater, T. Barraclough, I. Shield, N. Yates, S. C. Thain, I. S. Donnison, Influence of particle size on the analytical and chemical properties of two energy crops, Fuel 86, 60–72 (2007). DOI: 10.1016/j.fuel.2006.06.022.

E. Christoforou, P. A. Fokaides, A review of olive mill solid wastes to energy utilization techniques, Waste Manage. 49, 346–363 (2016).

DOI: org/10.1016/j.wasman.2016.01.012.

AOAC. Official Methods of Analysis. 18th ed. Association of Official Analytical Chemists International, Gaintersburg, MD, USA, 2006.

J. Trajković, J. Baras, M. Mirić, S. Šiler, Analize životnih namirinica, Tehnološko-metalurški fakultet, Beograd, Serbia, 1983, pp. 168–169.

FAO. Food Energy–methods of Analysis and Conversion Factors. Food and Agriculture Organization of the United Nations, Rome, Italy, 2003.

D. Hornero-Méndez, R. Gómez-Ladrón de Guevara, M. I. Ménguez-Mosquera, Carotenoid biosynthesis changes in five red pepper (Capsicum annuum L.) cultivars during ripening, cultivar selection for breeding, J. Agric. Food Chem. 48, 3857–3864 (2000).

J. Pino, M. González, L. Ceballos, A. R. Centurión-Yah, J. Trujillo-Aguirre, L. Latournerie-Moreno, E. Sauri-Duch, Characterization of total capsaicinoids, colour and volatile compounds of Habanero chilli pepper (Capsicum chinense Jack.) cultivars grown in Yucatan, Food Chem. 107, 1682–1686 (2007).

DOI: 10.1016/j.foodchem.2006.12.067.

Irish Standard I.S. EN ISO 11358-1:2014, Plas-tics−Thermogravimetry (TG) of Polymers − Part 1: General principles, 2014.

B. Krstic, A. Tepic, N. Nikolic, Dj. Gvozdenovic, M. Tomicic, Chemical variability of inedible fruit parts in pepper varieties (Capsicum annuum L.)., Bulg. J. Agric. Sci. 19(3), 490–496 (2013).

V. Rafajlovska, R. Slaveska-Raicki, J. Klopcevska, M. Srbinoska, Extraction of Oleoresin from Pungent Red Paprika under Different Conditions in: Mass Transfer in Chemical Engineering Processes, J. Markoš (Ed.), InTech, Rijeka, Croatia, 2011, pp. 111−132.

S. Kim, Y. T. Ha, J. Park, Characteristics of pigment composition and colour value by the difference of harvesting times in Korean red pepper varieties (Capsicum annuum L.), Int. J. Food Sci. Tech. 43(5), 915–920 (2008). DOI: 10.1111/j.1365-2621.2007.01542.x.

N. Zaki, A. Hakmaoi, A. Ouatmane, J. P. Fernandez-Trujillo, Quality characteristics of Moroccan sweet paprika (Capsicum annuum L.) at different sampling times, Food Sci. Technol. 33(3), 577–585 (2013).

DOI: org/10.1590/S0101-20612013005000072.

B. H. Gangadhar, K. R. Mishra, G. Pandian, S. W. Park, Comparative study of color, pungency, and biochemical composition in chili pepper (Capsicum annuum) under different light-emitting diode treatments, HortScience 47(12), 1729–1735 (2012).

R. Addala, M. Vasavada, J. Dong, S. Subramanian, Effect of storage conditions on rate of color degradation of paprika based products, J. Food Process Technol. 6(3), 1–6 (2015). DOI:10.4172/2157-7110.1000423.

M. D. Forero, C. E. Quijano, J. A. Pino, Volatile com-pounds of chile pepper (Capsicum annuum L. var. glabriusculum) at two ripening stages, Flavour Frag. J. 24(1), 25–30 (2009). DOI: 10.1002/ffj.1913.

M. Zimmermann, P. Schieberle, Important odorants of sweet bell pepper powder (Capsicum annuum cv. annuum): differences between samples of Hungarian and Moroccan origin, Eur. Food Res. Technol. 211, 175–180 (2000).

J. Pino, C. Fuentes, O. Barrios, Volatile constituents of Cachucha peppers (Capsicum chinense Jacq.) grown in Cuba, Food Chem. 125, 860–864 (2011).

DOI: 10.1016/j.foodchem.2010.08.073.

E. Fernándes-Garciá, A. Pérez-Gálvez, Flavoring Com-pounds in Red Pepper Fruits (Capsicum genus) and Processed Products, in: Handbook of Fruit and Vegetable Flavours, Y. H. Hui (Ed), Wiley & Sons, Inc., Hoboken, NJ, USA, 2010. pp. 909−934.

N. Ali, M. Saleem, M. Kamil, K. Shahzad, A. Chughtai, Thermo gravimetric study of Pakistani cotton & maize stalk using iso-conversional technique, Life Sci. J. 11(8), 382–387 (2014). http://www.lifesciencesite.com.

M. Poletto, L. Heitor, L. H. Ornaghi, A. J. Zattera, Native cellulose: Structure, characterization and thermal properties, Materials 7(9), 6105–6119 (2014).

DOI:10.3390/ma7096105.

D. Chen, M. Li, X. Zhu, TG-DSC method applied to drying characteristics and heat requirement of cotton stalk during drying, Heat Mass Transfer 48(12), 2087–2094 (2012). DOI: 10.1007/s00231-012-1050-6.

Y. Wang, Application of Fourier transform infrared mi¬cros¬copy (FTIR) and thermogravimetrical analysis (TGA) for quick identification of Chinese herb (Solanium lyratum), Plant Omics Journal (POJ) 5(6), 508–513 (2012).

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Published

2016-12-27

How to Cite

Simonovska, J., Škerget, M., Knez, Željko, Srbinoska, M., Kavrakovski, Z., Grozdanov, A., & Rafajlovska, V. (2016). Physicochemical characterization and bioactive compounds of stalk from hot fruits of Capsicum annuum L. Macedonian Journal of Chemistry and Chemical Engineering, 35(2), 199–208. https://doi.org/10.20450/mjcce.2016.944

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Section

Food Chemistry

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