ࡱ> ^`]` `bjbjss .bj%@@@@l4)!< > > > > > > $"h$\b FFFb  XXXF< XF< XXX pI ~@X  0)!X$B$X$XgrX\5b b 4$)!FFFF$@@ THE LIST OF CORECTIONS AND MODIFICATIONS REVIEWER B Abstract Reviewer comment: Would you clarify the name of the reagent resorcinol? Authors response: sulphapyridylazo resorcin has changed as sulphapyridylazo resorcinol in all text. Reviewer comment: analysis measurement Authors response: analysis has changed as measurement. Reviewer comment: subjecting FAAS measurement. Authors response: subjecting has changed as FAAS measurement. Reviewer comment: Edit!! To be able to determine the effects of experimental conditions such as pH, concentration of chelating agent and surfactant, equilibration temperature and time on cloud point extraction were studied. After optimization of the CPE conditions, an enrichment factor of 31, the detection limit of 1.64 g L"1were obtained from the calibration curve constructed in the range of 4 450 g L"1. The method was successfully applied to the determination of copper in tap water, stream water and rain water. The accuracy of the proposed method was examined to determine total copper in water certified reference material (SPS-WW1 wastewater). Authors response: This sentence has changed as Analytical parameters including pH, concentration of chelating agent and surfactant, equilibration temperature and time were optimized. The effects of the matrix ions on the recoveries of copper ions were also studied. The detection limits of 1.64 g L"1 along with enrichment factors of 31 for Cu were achieved. The method was successfully applied to the determination of copper in tap water, stream water, rain water and standard reference material. Introduction Reviewer comment: Acute toxicity of Cu toward marine organism should be mentioned and discussed. Authors response: Dissolved, reactive copper is toxic to marine plants and animals. Free ionic copper at concentration as low as 0.3 g L-1 decreases production in several species of oceanic phytoplankton. However, levels of copper exceed optimum values and thereby, they cause toxicity. was added into the text. New references [1] were added. Previous references numbers 1 and 2 has changed as 2 and 3, respectively. Previous references numbers [3-5] has changed as [4-6]. Reviewer comment: metal toxic elements Authors response: Metal has changed as toxic elements. Previous references numbers [6-12] has changed as [7-13]. Previous references number 13 has changed as 14. Reviewer comment: Addition addition Authors response: Addition has changed as addition. Previous references numbers [14-16] has changed as [15-17]. Previous references number 17 has changed as 18. Previous references number 18 has changed as 19. Reviewer comment: is are Authors response: is reported has changed as are reported. Previous references numbers [13-19] has changed as [14-20]. Reviewer comment: Chemical parameters The variables affecting the formation of a complex and extraction steps were optimized. Authors response: This sentence has changed as The main factors affecting the formation of a complex and extraction steps, such as pH, SPAR concentration, TritonX-100 concentration, phenol concentration, equilibration temperature and time were optimized. Experimental Reviewer comment: Vendor information for instrument and chemicals must be given consistently and completely: (company and country name). Authors response: 1,4-Dioxane (Merck, Darmstadt, Germany) was added. AG ZEEnit 700 (Germany) was added. Nve Water Bath Type NB9, Turkey) was added. Centrifuge Type NF400 (Turkey) was added. pH 720-pH meter (Germany) was added. Reviewer comment: Table 1 should be omitted well known routine instrumental parameters. Authors response: Mention data was removed from the manuscript Therefore, previous name of table 2 has changed as table 1. Reviewer comment: Omit this table 4. Mention data was removed from the manuscript Reviewer comment: Edit!! A 50 mL sample containing the analyte, 1.6 mL of 5% (w/v) Triton X-100, 1.4 mL of 5.010-4 mol L-1 SPAR as complexing reagent, borax buffer solution (10 mL) for an appropriate amount of pH value (pH=8.0) and 6 mL of 5% (w/v) phenol solution were placed into a centrifuge tube. Authors response: This sentence has changed as A typical cloud point extraction required following steps: a 50 mL sample containing the analyte, 1.6 mL of 5% (w/v) Triton X-100, 1.4 mL of 5.010-4 mol L-1 SPAR was adjusted to pH 8.0 with the addition borax buffer solution (10 mL) and 6 mL of 5% (w/v) phenol solution were placed into a centrifuge tube. Results and Discussion Reviewer comment: Edit!! The combination ratio was evaluated at the optimum pH (pH: 8.0) by mol ratio method, and it was found 1:1 (metal:ligand). Authors response: This sentence has changed as The molar ratio of copper to STAR in the complex was found to be 1:1. Previous references number 20 has changed as 21. Reviewer comment: Edit!! A set of CPE procedure with different concentrations of ligand in the range of 0.610-51.810-5 mol L-1 was performed to investigate the effect of the ligand on ions recovery. Authors response: This sentence has changed as The influence of the SPAR concentration on the CPE extraction of Cu was evaluated in the concentration range of 0.610-51.810-5 mol L-1. Previous references number 21 has changed as 22. Previous references numbers [19,22, 24] has changed as [20,23,25]. Reviewer comment: SDS?? Authors response: SDS has changed as sodium dodecyl sulphate. Previous references number 23 has changed as 24. Previous references numbers [5, 13] has changed as [6, 14]. Reviewer comment: Edit!! For this reason, phenol solution was used in this study, and its effect in the concentration range of 0.20.8% phenol (w/v) was studied. A phenol concentration of 0.6% (w/v) was selected to induce phase separation and to improve extraction efficiency. As it is shown in figure 4 the recovery was increased up to phenol concentration of 0.6% (w/v) and remained constant. Authors response: This sentence has changed as Therefore, its effect in the concentration range of 0.2-0.8% phenol (w/v) was studied. The optimum phenol concentration was found 0.6%(w/v). Figure 4 was removed from the manuscript. Figure 4 was removed from the manuscript. Reviewer comment: Edit!! Our results show that the optimum volume of methanol is 0.4 mL. Smaller added volumes of acidified methanol were not tested because in which case the signal is decreased due to inconvenient features of solution for aspiration and nebulizer. Authors response: This sentence has changed as Our results show that the optimum volume of methanol is 0.4 mL. Smaller added volumes of acidified methanol were not tested because in this case it was not possible to quantitatively transfer the rich phase from test tubes to the graduated tubes and measuring the absorbance. Effect of diverse ions section was added sentence Table 1. in these experiments, solutions containing 26.5 g L-1 of Cu and the interfering ions were treated according to the recommended procedure. Reviewer comment: Please present recovery in a range e.g. 973 or >99 Authors response: Previous name of table 2 has changed as table 1. Table 1 caption and to content was revised. Reviewer comment: What about the calibration method and calibration solutions please describe. Performance characteristics of the method were obtained by processing standard solution of copper. The calibration graph using CPE preconcentration system for copper was linear in the range of 4450 g L"1 of Cu (II) with correlation coefficient of 0.994. The regression equation was A=0.00507 C + 0.001. Author s response: This sentence has changed as  Calibration graphs were obtained by the preconcentration of 50 mL of standard solutions in the presence of 0.16% Triton X-100 in medium at pH 8.0. The samples were introduced into the flame by conventional aspiration following the addition in 0.4 mL of 0.1 mol L-1 HNO3, methanol solution in. Copper can be determined in the range of 4 450 g L"1 of Cu (II) w67?@AQRS        % & / 0 1 2 I ž̠ř̃|umeu]Uhk*h45hk*hp5hk*hF7hk*hFn7 hk*hFn hk*h4 hk*h@ghk*h1:5 hk*h.' hk*h,-hk*hF5hk*hk*5 hk*h%+ hk*h1: hk*hF hk*hYhHhk*h@g5 hk*hiVw hk*h(%|hk*h%+5>*hk*h-5>*hk*h@g5>*!+7@AS  0 X k OPd6EFZ $dha$gdF`I J U V X i j k l m w x   MNOPXbcd46սնܶ㯨|| hk*h4:hk*h45 hk*hpHhk*h(%|H* hk*hb hk*h(%| hk*h hk*h. hk*hfvghk*hk*5hk*h47hk*hFn7 hk*hFn hk*h4 hk*h@ghk*h@g5 hk*h.' hk*hF06CDEFWXYZkp&WhijkĽĽˑˮىĂĂ{t hk*h~d\ hk*h8>s hk*h@hk*hriI5hk*h&7 hk*h$l hk*h>0- hk*h>Ahk*hk*5 hk*h 8 hk*hy hk*h0L hk*h& hk*h@ghk*h@g5 hk*h d hk*hG^ hk*h%+hk*h%+5>*-O&W~%Vqrs$dh1$G$[$\$a$gdFm$ $dha$gdFkst~%TVghiklqyowqʼاџؑاу|ا| hk*h- hk*h3 hk*h[l hk*h 8 hk*h5hk*h57 hk*h@g hk*h~ hk*h hk*hp hk*h~d\ hk*h hk*h0Lhk*h@g5hk*hk*5 hk*h&hk*h0L7hk*h&7/qrst +DQtJ\uĶssfYYYL?hk*h 8OJQJ^Jhk*hOJQJ^Jhk*h9OJQJ^Jhk*h*OJQJ^Jhk*h-5>*OJQJ^J hk*hiVw hk*h: QtJ\.6N`cw $dha$gdF$dh1$G$[$\$a$gdFm$,-.6MN`˾~wpibZbZbSKbZbZbhk*hp5 hk*h hk*hqH* hk*hq hk*h+ hk*h0L hk*h@ghk*h@g5 hk*h0LPJmH nHsH tHhk*hE'OJQJ^Jhk*h[lOJQJ^Jhk*h0LOJQJ^Jhk*h+OJQJ^Jhk*h@gOJQJ^Jhk*h@g5OJQJ^Jhk*h 8OJQJ^Jhk*hAOJQJ^J8Wckw '(~ !!! !"!'!(!)!ȹȪȣϜ~~ww hk*h0L hk*hvhk*huH*hk*hMAH* hk*hMA hk*hu hk*h%7 hk*hC6Whk*hp5hk*hk*5 hk*h 8 hk*h#C hk*h+ hk*h@ghk*h@g5hk*hAR5>*hk*hiVw5>* hk*h d. ' !!!)!-B-J-L---.PHQLQPQRQTQvQxQzQ;Ͷ۠ۇyl_hk*hpOJQJ^Jhk*h@gOJQJ^Jhk*h@g5OJQJ^J hk*hqUhk*hH*hk*hH* hk*h hk*hphk*hp5hk*hk*5hk*h+H* hk*h hk*h+ hk*hT hk*hA hk*h0L hk*hn hk*h@ghk*h@g5"ith correlation coefficient of 0.994. The regression equation was A=0.00507 C + 0.001, where A is the absorbance and C is the copper concentration in solution (g L"1). Reviewer comment: All data have been already presented in the text. No need from this table 3. Authors response: Mention data was removed in the text. Therefore, previous name of table 5 has changed as table 2. Reviewer comment: Omit this Table 4 Authors response: Mention data was removed in the text. Previous name of table 5 has changed as table 2 and to content was revised. Reviewer comment: The result should be compared with literature values such as detection limit, RSD, preconcentration factor. Authors response: Comparison table 3 was added. Conclusion Reviewer comment: The fundamental CPE of Cu (II) from environmental samples coupled with FAAS was investigated.?? Authors response: This sentence has changed as The surfactant-rich phase could be introduced into the nebulizer of a FAAS after dilution with acidified methanol. Table 3 shows a comparison of the method considered with those found in literature. was added into the text. Reviewer comment: No connection between RSD and versatility of the method The low RSD value of real sample analysis is evidence for the verzQQR RRRRR+R7RBRDRERbRhRyRRRRRRRRRRRRRRRRʽ󎇀yrkc[rTyM hk*h~d\ hk*h3\Xhk*hp5hk*hk*5 hk*h hk*h;: hk*h0L hk*h hk*h@ghk*h@g5hk*hsOJQJ^Jhk*hPOJQJ^Jhk*h0LOJQJ^Jhk*hpOJQJ^Jhk*hp5OJQJ^Jhk*hk*5OJQJ^Jhk*hOJQJ^Jhk*hNOOJQJ^JRRR S S-S>S?S@SASSSSSSSSSS\T]T^TfT˾{kc[SLEL=hk*hk*5 hk*hr hk*hAhk*h0L5hk*h 5hk*h@g5hk*hq65>*OJQJ^Jhk*hUOJQJ^Jhk*hp5OJQJ^Jhk*hk*5OJQJ^Jhk*hsOJQJ^Jhk*h0LOJQJ^Jhk*h OJQJ^Jhk*h@gOJQJ^Jhk*h@g5OJQJ^Jhk*h3\XOJQJ^Jhk*h.gOJQJ^J^TqTUrUUU&`9``` $dha$gd0L $dha$gdF fTqTUUqUrUUUUUV``%`&`.`8`9`V`````žۼۥۓhk*h /mHsH hk*hDk hk*h\hk*h*@5hk*hk*5U hk*h0L hk*h hk*h@ghk*h@g5 hk*h*@ hk*h( hk*h%+ hk*hAhk*hp5satility of methods for real sample. Authors response: This sentence has changed as This procedure is a good alternative to accurate determination of copper in environmental water samples. ,1h. 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