TY - JOUR AU - Stojanovska Dimzoska, Biljana AU - Hajrulai Musliu, Zehra AU - Uzunov, Risto AU - Angeleska, Aleksandra AU - Blagoevska, Katerina AU - Crceva Nikolovska, Radmila AU - Ilievska, Gordana AU - Dimitrieska Stojkovic, Elizabeta PY - 2023/06/12 Y2 - 2024/03/28 TI - Study on the effectiveness of a multi-toxin immunoaffinity clean-up for reliable cost-effective HPLC-FLD analysis of mycotoxins in corn-based foods JF - Macedonian Journal of Chemistry and Chemical Engineering JA - Maced. J. Chem. Chem. Eng. VL - 41 IS - 1 SE - Analytical Chemistry DO - 10.20450/mjcce.2022.2422 UR - https://mjcce.org.mk/index.php/MJCCE/article/view/2422 SP - 77-88 AB - <p>A different, reliable, and cost-effective strategy for the analysis of aflatoxins, ochratoxin A, and zearalenone in corn-based foods was proposed, including one multi-toxin immunoaffinity column (IAC) sample preparation and three different high-performance liquid chromatography fluorescence detection methods. The analytical procedures were tested and verified, keeping in mind their occurrence at trace levels in corn-based foods. With the validation of the proposed multi-toxin IAC methodology and comparison of the performance characteristics with methods using a single-toxin IAC, we confirmed the reliability of the multi-toxin IAC procedure versus the single-toxin IAC. The methods were validated by revealing satisfactory performance characteristics; for example, the obtained values of limit of detection were significantly lower than the maximum limits for all mycotoxins of concern. In addition, the recovery values were between 70.9 % and 106.1 % for all mycotoxins of interest, with precision values lower than 10.5 %.</p><p> </p> ER -