TY - JOUR AU - Dimitrieska-Stojkovik, Elizabeta AU - Ilievska, Gordana AU - Stojanovska-Dimzoska, Biljana AU - Uzunov, Risto AU - Hajrulai-Musliu, Zehra PY - 2014/12/01 Y2 - 2024/03/29 TI - Study on the method performances for screening and confirmatory analysis of aflatoxin M1 in raw milk according to the mycotoxin regulative requirements JF - Macedonian Journal of Chemistry and Chemical Engineering JA - Maced. J. Chem. Chem. Eng. VL - 33 IS - 2 SE - Food Chemistry DO - 10.20450/mjcce.2014.486 UR - https://mjcce.org.mk/index.php/MJCCE/article/view/mjcce.2014.486 SP - 217-226 AB - <p>Screening and confirmatory methods for Aflatoxin M<sub>1</sub> (AFM<sub>1</sub>) analysis were optimized and studied regarding the European Decision 2002/657/EC requirements. The recoveries of AFM<sub>1 </sub>from the spiked samples at levels between 0.025 and 0.075 µg kg<sup>-1</sup> were 99.6-110.3 % with obtained precision 10.5-13.4 %, confirming the immunochemical method precision and reliability for intensive surveillance studies. According to the regulatory requirements, for confirmation of the obtained positives from the screening, the reference high-performance liquid chromatographic method was evaluated in reference to the performance criteria. The obtained between-day recovery was in the range 67.2-72.8 %, and the precision range 5.4-9.1 %. 205 positives from the screening were subjected to confirmatory analysis. The comparison of the data obtained from the immunoassay and chromatographic method (<em>R</em><sup>2</sup>=0.764) revealed a slight overestimation of the screening method, but no cases of false-negative determinations occurred.</p> ER -