Population Diversity of n–Alkanes in the Needle Cuticular Wax of Relicts Pinus heldreichii and P. peuce from the Scardo-Pindic Mountains

Authors

  • Biljana M. Nikolić Institute of Forestry, 11000 Belgrade http://orcid.org/0000-0002-2436-8294
  • Iris Đorđević Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade
  • Marina Todosijević Institute for Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade
  • Zorica Mitić University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology, Višegradska 33, 18000 Niš
  • Srdjan Bojović Institute for Biological Research “Siniša Stanković”, University of Belgrade, Boulevard Despota Stefana 142, 11060 Belgrade
  • Petar D. Marin Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, University of Belgrade, Studentski trg 16, 11000 Belgrade

DOI:

https://doi.org/10.20450/mjcce.2020.1951

Keywords:

Pinus heldreichii, P. peuce, n-alkanes, diversity, Principle component analysis (PCA), Cluster analysis

Abstract

The variability of n-alkanes in the needle cuticular wax of Pinus heldreichii and P. peuce in two natural populations from the Scardo-Pindic mountains was investigated for the first time. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC–MS) analyses of two-year-old needles were performed using an Agilent 7890A GC equipped with an inert 5975C XL EI/CI mass spectrometer detector (MSD) and a flame ionization detector (FID) connected by a capillary flow technology 2-way splitter with make-up. An HP-5MS capillary column was used. n-Alkanes ranged from C19 to C33. In P. heldreichii the most abundant were C23, C25 and C27 (16.3, 15.6 and 12.8% on average, respectively), while in P. peuce they were C29, C25, and C27 (16.5, 15.3 and 13.5% on average, resp.). Mid-chain and long-chain n-alkanes prevailed in both species. Principle component analysis (PCA) and Cluster analyses of five and six n-alkanes, respectively, showed divergence of the Scardo-Pindic populations from the Dinaric ones.

Author Biography

Biljana M. Nikolić, Institute of Forestry, 11000 Belgrade

Departement of genetics and physiology of forest trees

References

M. Vidaković, Četinjače. Morfologija i varijabilnost, JAZU i Sveučilišna naklada Liber, Zagreb, 1982.

B. Nikolić, M. Ristić, S. Bojović, V. Matevski, Z. Krivošej, P. D. Marin, Essential-oil composition of the needles collected from natural populations of Macedonian pine (Pinus peuce Griseb.) from the Scardo-Pindic moun-tain system. Chem. Biodivers., 11, 934–948 (2014). DOI: https://doi.org/10.1002/cbdv.201300343

B. Nikolić, M. Ristić, S. Bojović, Z. Krivošej, V. Matev-ski, P. D. Marin, Population variability of essential oils of Pinus heldreichii from the Scardo-Pindic Mountains Ošljak and Galičica. Chem. Biodivers., 12, 295–308 (2015). DOI: https://doi.org/10.1002/cbdv.201400135

T. G. Knight, M. A. B. Wallwork, M. Sedgley, Leaf epi-cuticular wax and cuticle ultrastructure of four Eucalyptus species and their hybrids. Int. J. Plant Sci., 165, 27–36 (2004).

DOI: https://www.jstor.org/stable/10.1086/380744

Z. S. Mitić, B. K. Zlatković, S. Č. Jovanović, J. S. Ni-kolić, B. M. Nikolić, G. S. Stojanović, P. D. Marin, Di-versity of needle n-alkanes, primary alcohols and diter-penes in Balkan and Charpathian native populations of Pinus nigra J.F. Arnold. Biochem. Syst. Ecol. 80, 46–54 (2018). http://biore.bio.bg.ac.rs/handle/123456789/2914

C. Lutz, V. Heinzmann, P. G. Gülz, Surface structures and epicuticular wax composition of spruce needles after long-term treatment with ozone and acid mist. Environ. Pollut, 64, 313–322 (1990).

DOI: https://doi.org/10.1016/0269-7491(90)90053-F

R. S. Dodd, M. M. Poveda, Environmental gradients and population divergence contribute to variation in cuticular wax composition in Juniperus communis. Biochem. Syst. Ecol. 3, 1257–1270 (2003).

DOI:10.1016/S0305-1978(03)00031-0

M. Maffei, S. Badino, S. Rossi, Chemotaxonomic signif-icance of leaf wax n-alkanes in the Pinales (Coniferales). J. Biol. Res. – Thessalon. 1, 3–19. (2004).

B. Nikolić, V. Tešević, I. Đorđević, M. Jadranin, M, Todosijević, S. Bojović, P.D. Marin, Chemodiversity of nonacosan‐10‐ol and n‐alkanes in the needle wax of Pi-nus heldreichii. Chem. Biodivers. 9, 80–90 (2012a), DOI: 10.1002/cbdv.201100179

B. Nikolić, V. Tešević, I. Đorđević, M. Todosijević, M. Jadranin, S. Bojović, P. D. Marin, Population variability of nonacosan-10-ol and n-alkanes in needle cuticular waxes of Macedonian Pine (Pinus peuce Griseb.). Chem. Biodivers. 9, 1155–1165 (2012b).

DOI: 10.1002/cbdv.201100316

B. Nikolić, M. Ristić, S. Bojović, V. Matevski, Z. Krivošej, P. D. Marin, Essential-oil composition of the needles collected from natural populations of Macedonian pine (Pinus peuce Griseb.) from the Scardo-Pindic moun-tain system. Chem. Biodivers. 11, 934–948 (2014). DOI: 10.1002/cbdv.201300343

B. Nikolić, M. Ristić, S. Bojović, P. D. Marin, Popula-tion variability of essential oils of Pinus heldreichii from the Scardo-Pindic mountains. Chem. Biodivers. 12, 295–308 (2015). DOI: 10.1002/cbdv. 201400135

M. R. M. Mimura, M. L. F. Salatino, A. Salatino, J. F. A. Baumgratz, Alkanes from epicuticular waxes of Huberia species: taxonomic implications. Biochem. Syst. Ecol. 26,581–588 (1998).

DOI: https://doi.org/10.1016/S0305-1978(97)00131-2

H.Van Den Dool, P. D. Kratz, A generalization of the retention index system including linear temperature pro-grammed gas-liquid partition chromatography. J. Chromatogr. 11, 463–471 (1963).

DOI: https://doi.org/10.1016/S0021-9673(01)80947-X

M. A. Mazurek, B. R. T. Simoneit, Caracterization of biogenic and petroleum-derived organic matter in aerosols over remote, rural and urban areas in: Identification and Analysis of Organic Pollutants in Air. (Ed.) Keith, Ann. Arbor, Science / Butterworth Publishers, Boston, 1984, pp. 353–370.

J. Poynter, G. Eglinton, Molecular composition of three sediments from Hole 717C: the Bengal Fan. J. R. Cochran and D.A.V. Stow (Eds), Proc. ODP Sci. Re-sults, 116, 1990, pp. 155–161.

E. E. Bray, E. D. Evans, Distribution of n-paraffins as a clue to recognition of source beds. Geochim. Cosmochim. Acta 22, 2–15 (1961).

DOI: https://doi.org/10.1016/0016-7037(61)90069-2

T. K. Kuhn, E. S. Krull, A. Bowater, K. Grice, G. Gleixner, The occurrence of short chain n-alkanes with an even over odd predominance in higher plants and soils. Org. Geochem. 41, 88–95 (2010).

DOI: https://doi.org/10.1016/j.orggeochem.2009.08.003

D. R. Oros, L. J. Standley, X. Chen, B. R. Simoneit, Epicuticular wax compositions of predominant conifers of western North America. Z. Naturforsch. C. 54, 17–24. (1999). DOI: 10.1515/znc-1999-1-205

Y. Chikaraishi, H. Naraoka, Compound-specific δ2H–δ13C analyses of n-alkanes extracted from terrestrial and aquatic plants. Phytochemistry 63, 361–371 (2003).

DOI: https://doi.org/10.1016/S0031-9422(02)00749-5

B. Nikolić, M. Ristić, S. Bojović, P. D. Marin, Variability of the needle essential oils of Pinus peuce from different populations in Montenegro and Serbia, Chem. Biodivers. 5, 1377–1388 (2009).

DOI: http://dx.doi.org/10.1080/11263504.2014.1000999

M. J. Lockheart, I. Poole, P.F.Van Bergen, R.P. Evershed, Leaf carbon isotope composition and stomatal characters: important considerations for palaeoclimate re-construction. Org. Geochem. 29, 1003–1008 (1998).

DOI: https://doi.org/10.1016/S0146-6380(98)00168-5

N. C. Arens, A. H. Jahren, R. Amundson, Can C3 plants faithfully record the carbon isotopic composition of at-mospheric dioxide? Paleobiology, 26, 137–164 (2000).

DOI: https://doi.org/10.1666/0094-8373(2000)026<0137:C CPFRT>2.0.CO;2

A. Vogts, H. Moossen, F. Rommerskirchen, J. Rullkötter, Distribution patterns and stable carbon isotop-ic composition of alkanes and alkan-1-ols from plant waxes of African rain forest and savanna C3 species. Org. Geochem. 40, 1037–1054 (2009).

DOI:10.1016/j.orggeochem.2009.07.011

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Published

2020-06-11

How to Cite

Nikolić, B. M., Đorđević, I., Todosijević, M., Mitić, Z., Bojović, S., & Marin, P. D. (2020). Population Diversity of n–Alkanes in the Needle Cuticular Wax of Relicts Pinus heldreichii and P. peuce from the Scardo-Pindic Mountains. Macedonian Journal of Chemistry and Chemical Engineering, 39(1), 41–48. https://doi.org/10.20450/mjcce.2020.1951

Issue

Vol. 39 No. 1 (2020)

Section

Natural Products

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