Microwave assisted solvent-free mannich bases: synthesis, characterization and effects of these compounds on HCA I and HCA II isozymes

Authors

  • Bülent Büyükkıdan Department of Chemistry, Faculty of Arts and Sciences, Kutahya Dumlupınar University, 43100 Kütahya, Turkey http://orcid.org/0000-0001-9619-3246
  • Nurgün Büyükkıdan Department of Chemistry, Faculty of Arts and Sciences, Kutahya Dumlupınar University, 43100 Kütahya
  • Metin Bülbül Department of Biohemistry, Faculty of Arts and Sciences, Kutahya Dumlupınar University, 43100 Kütahya
  • Melek Yılmaz Department of Chemistry, Faculty of Arts and Sciences, Kutahya Dumlupınar University, 43100 Kütahya
  • Evren Derrun Arslanbay Department of Chemistry, Faculty of Arts and Sciences, Kutahya Dumlupınar University, 43100 Kütahya
  • Ekrem Tunca Department of Biohemistry, Faculty of Arts and Sciences, Kutahya Dumlupınar University, 43100 Kütahya

DOI:

https://doi.org/10.20450/mjcce.2021.1968

Keywords:

Activator, carbonic anhydrase, glaucoma, isozyme

Abstract

Mannich bases (2a–d) of aromatic amines were synthesized by using a conventional microwave technique under solvent-free conditions and characterized by IR and NMR (1H and 13C) and elemental analysis. The inhibitory effects of the synthesized Mannich bases were examined in vitro by using hydratase and esterase assays on carbonic anhydrase I and II isozymes (hCA, EC 4.2.1.1) purified from human erythrocyte cells. Acetazolamide was used as the control compound. The values of IC50, the half-maximum inhibitory concentration, were found for hydratase and esterase activities. Only two compounds (2b and 2d) exhibited poor hCA I and hCA II inhibition effects on esterase activity. In contrast, compounds 2a and 2c could be used as carbonic anhydrase activators as a result of the increased esterase activity of hCA I and hCA II isozymes.

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Published

2021-05-12

How to Cite

Büyükkıdan, B., Büyükkıdan, N., Bülbül, M., Yılmaz, M., Arslanbay, E. D., & Tunca, E. (2021). Microwave assisted solvent-free mannich bases: synthesis, characterization and effects of these compounds on HCA I and HCA II isozymes. Macedonian Journal of Chemistry and Chemical Engineering, 40(1), 51–56. https://doi.org/10.20450/mjcce.2021.1968

Issue

Vol. 40 No. 1 (2021)

Section

Organic Chemistry

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