Application of reliable cost-effective strategy for analysis of mycotoxins in corn-based foods with HPLC-FLD after multi-toxin immunoaffinity clean-up

Authors

  • Biljana Stojanovska Dimzoska Food Institute, Faculty of Veterinary Medicine Skopje http://orcid.org/0000-0003-1688-6179
  • Zehra Hajrulai Musliu Food Institute, Faculty of Veterinary Medicine Skopje
  • Risto Uzunov Food Institute, Faculty of Veterinary Medicine Skopje
  • Aleksandra Angeleska Food Institute, Faculty of Veterinary Medicine Skopje
  • Katerina Blagoevska Food Institute, Faculty of Veterinary Medicine Skopje
  • Radmila Crceva Nikolovska Food Institute, Faculty of Veterinary Medicine Skopje
  • Gordana Ilievska Food Institute, Faculty of Veterinary Medicine Skopje
  • Elizabeta Dimitrieska Stojkovic Food Institute, Faculty of Veterinary Medicine Skopje

DOI:

https://doi.org/10.20450/mjcce.2022.2422

Keywords:

mycotoxins, multi-toxin IAC, HPLC-FLD, validation

Abstract

A different, reliable, and cost-effective strategy for the analysis of aflatoxins, ochratoxin A, and zearalenone in corn-based foods was proposed, including one multi-toxin immunoaffinity column (IAC) sample preparation and three different high-performance liquid chromatography fluorescence detection methods. The analytical procedures were tested and verified, keeping in mind their occurrence at trace levels in corn-based foods. With the validation of the proposed multi-toxin IAC methodology and comparison of the performance characteristics with methods using a single-toxin IAC, we confirmed the reliability of the multi-toxin IAC procedure versus the single-toxin IAC. The methods were validated by revealing satisfactory performance characteristics; for example, the obtained values of limit of detection were significantly lower than the maximum limits for all mycotoxins of concern. In addition, the recovery values were between 70.9 % and 106.1 % for all mycotoxins of interest, with precision values lower than 10.5 %.

 

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Published

2022-06-22

How to Cite

Stojanovska Dimzoska, B., Hajrulai Musliu, Z., Uzunov, R., Angeleska, A., Blagoevska, K., Crceva Nikolovska, R., Ilievska, G., & Dimitrieska Stojkovic, E. (2022). Application of reliable cost-effective strategy for analysis of mycotoxins in corn-based foods with HPLC-FLD after multi-toxin immunoaffinity clean-up. Macedonian Journal of Chemistry and Chemical Engineering, 41(1). https://doi.org/10.20450/mjcce.2022.2422

Issue

Section

Analytical Chemistry

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