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Effects of Flavobacterium psychrophilum extracellular products and lipopolysaccharide antigens on the antioxidant enzyme system activity of rainbow trout (Oncorhynchus mykiss) fry

Authors

  • Muammer Kirici Department of Veterinary Health, Food Agriculture and Livestock Vocational School, Bingöl University, 12000, Bingöl, Turkey https://orcid.org/0000-0003-1888-4388
  • Mahinur Kirici Bingöl University, Faculty of Arts and Science, Department of Chemistry, Bingöl
  • Mehmet Resit Taysi Department of Fisheries, Faculty of Agriculture, Bingöl University, Bingöl
  • Unal Ispir Malatya Turgut Özal University, Doğanşehir Vahap Küçük Vocational School, Department of Aqua-culture, Malatya

DOI:

https://doi.org/10.20450/mjcce.2022.2482

Keywords:

Flavobacterium psychrophilum, fish, antioxidant enzymes, lipopolysaccharide, extracellular products

Abstract

The aim of this study was to investigate the response of different doses of lipopolysaccharide (LPS) and extracellular product (ECP) antigens (25, 50, 100, 250, and 500 µg) obtained from Flavobacterium psychrophilum to changes in oxidative stress parameters (catalase [CAT], superoxide dismutase [SOD], glutathione peroxidase [GPx], glucose 6-phosphate dehydrogenase [G6PD], glutathione reductase [GR], and malondialdehyde [MDA]) in juvenile rainbow trout. For this purpose, 6 different doses of F. psychrophilum (102–106) were applied to the fish, and the lethal dose 50 % (LD50) value was determined as 1.33·104. Changes in MDA levels and antioxidant enzymes were determined by applying 25 % of the LD50 value to fish for 14 days. Accordingly, while F. psychrophilum increased GR, G6PD, GPx, and SOD activity, it decreased MDA levels and CAT activity. Initially, 25, 50, 100, 250, and 500 µg doses of ECP and LPS were applied to the fish; after 21 days, a 25 % dose of LD50 was applied to these fish. Fourteen days later, the fish were taken, and the changes in MDA levels and antioxidant enzymes were examined. As a result, it was determined that while ECP and LPS application decreased GR, G6PD, GPx, and SOD activity, there was an increase in MDA levels and CAT activity.

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Published

2022-12-19

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How to Cite

Kirici, M., Kirici, M., Taysi, M. R., & Ispir, U. (2022). Effects of Flavobacterium psychrophilum extracellular products and lipopolysaccharide antigens on the antioxidant enzyme system activity of rainbow trout (Oncorhynchus mykiss) fry. Macedonian Journal of Chemistry and Chemical Engineering, 41(2). https://doi.org/10.20450/mjcce.2022.2482

Issue

Vol. 41 No. 2 (2022)

Section

Biochemistry

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Copyright (c) 2022 Muammer KIRICI, Mahinur KIRICI, Mehmet Reşit TAYSI, Ünal İSPİR

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