Free and potentially volatile monoterpenes in grape varieties from the Republic of Macedonia

Donka Doneva-Šapčeska, Aco Dimitrovski, Trajko Bojadžiev, Goran Milanov, Borimir Vojnovski


The total content of free (FVT) and potentially volatile terpenes (PVT) present in the grape varieties Muscat Hamburg, Muscat Italia, Italian Riesling, Chardonnay, Vranec and Cabernet Sauvignon was determined by using a rapid distillation-colorimetric method. The assays were performed on samples of mature grapes. The FVT and PVT correlated with the recognized aroma characteristics of the cultivars examined. The level of free monoterpenes in the skin, the pulp, and the juice from the Muscat Italia grape was lower than the level of bound monoterpenes. Most of the free monoterpenes were located in the skin. However, the calculated contents of the FVT and the PVT present in the skin were much lower when expressed on the whole berry. Additionally, the contents of FVT and PVT in grape waste obtained after pressing was examined. The quantity of potentially volatile monoterpene components determined in the grape varieties investigated opens up the possibility of enhanceing the free monoterpene contents in the wine-making process.


grape; free volatile terpenes; potentially volatile terpenes

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J. Marais, Terpenes in the aroma of grapes: A review, S. Afr. J. Enol. Vitic., 4, 49–60 (1983).

C. R. Strauss, B. Wilson, P. R. Gooley, P. J. Williams, Role of monoterpenes in grape and wine flavor, in Biogeneration of aromas, T. Parliament, R. Croteau (eds.), ACS Symposium series 317, American Chemical Society, Washington, 1986, pp. 222–242.

J. J. Mateo, M. Jiménez, Monoterpenes in grape juice and wines, J. Chromatogr. A, 881, 557–567 (2000).

Y. Z. Gunata, C. L. Bayonove, R. L. Baumes, R. E. Cordonnier, The aroma of grapes, I. Extraction and determination of free and glycosidically bound fractions of some grape aroma components, J. Chromatogr., 331, 89–90 (1985).

Y. Z. Gunata, C. Bayonove, R. Baumes, R. E. Cordonnier, The aroma of grapes, II. The localization and evolution of free and bound fractions of some grape aroma components c.v. Muscat during development and maturation, J. Sci. Food Agric., 36, 857–862 (1985).

O. Shoseyov, B. A. Bravdo, D. Siegel, A. Goldman, S. Cohen, L. Shoseyov, R. Ikan, Immobilized endo-ßglucosidase enriches flavor of wine and passion fruit juice, J. Agric. Food Chem., 38, 1387–1390 (1990).

M. A. Sefton, I. L. Francis, P. J. Williams, The volatile composition of Chardonnay juices: A study by flavor precursor analysis, Am. J. Enol. Vitic., 44, 359–370 (1993).

A. Ganga, F. Piñaga, A. Querol, S. Vallés, D. Ramón, Cell-wall degrading enzymes in the release of grape aroma precursors, Food Sci. Technol. Int., 7, 83–87 (2001).

S. K. Park, J. C. Morrison, D. O. Adams, A. C. Noble, Distribution of free and glycosidically bound monoterpenes in the skin and mesocarp of Muscat of Alexandria grapes during development, J. Agric. Food Chem., 39, 514–518 (1991).

B. G. Coombe, M. G. McCarthy, Identification and naming of the inception of aroma development in ripening grape berries, Austral. J. Grape Wine Res., 3, 18–20 (1997).

E. García, J. L. Chacón, J. Martínez, P. M. Izquierdo, Changes in volatile compounds during ripening in grapes of Airén, Macabeo and Chardonnay white varieties grown in La Mancha region (Spain), Food Sci. Technol. Int., 9, 33–41 (2003).

W. D. Edinger, Enhancement of varietal character through liberation of bound monoterpenes. H.R.I.O. Enol. Lab Tech. Note No. 8 (1988).

A. G. Reynolds, D. A Wardle, M. Dever. Terpene response to pressing, harvest date, and skin contact in Vitis vinifera, Hort Science, 28, 920–924 (1993).

E. Falque, E. Fernandez, Effects of different skin contact times on Treixadura wine composition, Am. J. Enol. Vitic., 47, 309–312 (1996).

S. Genovés, J. V. Gil, S. Vallés, J. A. Casas, P. Manzanares, Assessment of the aromatic potential of palomino fino grape must using glycosidases, Am. J. Enol. Vitic., 56, 188–191 (2005).

E. Dimitriadis, P. J. Williams, The development and use of a rapid analytical technique for estimation of free and potentially volatile monoterpene flavorants of grapes, Am. J. Enol. Vitic., 35, 66–67 (1984).

D. Doneva-Šap_eska, Influence of the free and glycosidically bound monoterpenes on the grape and wine aroma, Ph.D. thesis, University Ss. Cyril and Methodius, Skopje, 2005.

A. Rapp, Wine aroma substances from gas chromatographic analysis, in Wine analysis, Vol. 6, H. F. Linskern and J. F. Jackson (eds.), Springer-Verlag, Berlin, Heidelberg, 1988, pp. 29–66.

R. Strauss, B. Wilson, P. R. Gooley, P. J. Williams, Novel monoterpene diols and diol glycosides in vitis vinifera grapes, J. Agric. Food Chem., 36, 569–573 (1989).

V. Ferreira, R. López, J. Cacho, Quantitative determination of the odorants of young red wines from different grape varieties, J. Sci. Food Agric., 80, 1659–1667 (2000).

G. Versini, A. Dalla Serra, M. Falcetti, Rôle du clone, du millésime et de l’époque de la récolte sur le potentiel aromatique du raisin de Chardonnay, Rev. Oenol. Techn. Viti-vini. OEEnol, 65, 19–23 (1992).

G. Wilson, C. R. Strauss, P. J. Williams, The distribution of free and glycosidically-bound monoterpenes among skin, juice, and pulp fractions of some white grape varieties, Am. J. Enol. Vitic., 37, 107–111 (1986).

S. Park, A. Noble, Monoterpenes and monoterpene glycosides in wine aromas, in Beer and wine production, B. H. Gump (ed.), ACS Series 536, American Chemical Society, Washington, 1993, pp. 99–109.

B. Girard, L. Fukamoto, G. Mazza, P. Delaquis, B. Ewert, Volatile terpene constituents in maturing Gewürztraminer grapes from British Columbia, Am. J. Enol. Vitic., 53, 99–109 (2002).


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