Voltammetric behavior and determination of the bronchodilator doxofylline at a boron-doped diamond electrode

Sachin Saxena, Ratnanjali Shrivastava, Soami P. Satsangee


The electrochemical behavior of the asthma drug doxofylline was studied at a boron-doped diamond electrode in BR buffer (pH 3.8) by square-wave voltammetry. Cyclic voltammetry revealed the oxidation of doxofylline as an irreversible diffusion-controlled process. The oxidation mechanism was proposed and the drug was studied in the presence of solubilized systems like Tween-20 (T-20), sodium dodecyl sulfate (SDS), Triton X-100 (TTN), dioxane (DO), dimethylformamide (DMF), diethyl ether (DEE), benzene and methanol (MeOH). Comparison of the current responses in the solution system explained the sensitivity of the boron-doped diamond electrode (BDDE) with a three-fold increase in the current value compared to that of a glassy carbon electrode (GCE) at a fixed concentration of doxofylline. Peak current values varied linearly with increasing concentration and the LOD and LOQ were found to be 3.48 and 10.56 µg/ml, respectively. This method was employed to investigate doxofylline in the pharmaceutical formulation.


Doxofylline, BDDE, Voltammetry, pharmaceutical formulation.

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X. Kang, J. Wang, H.Wu, J. Liu, I.A.Aksay, Y. Lin, A graphene based electrochemical sensor for sensitive detection of Paracetamol, Talanta, 81, 754–759 (2010).

F.G.Marc and C.Paul, Efficacy and safety of doxofylline compared to theophylline in chronic reversible asthma -- a double-blind randomized placebo-controlled multicentre clinical trial. Med. Sci. Monit., 8(4), CR297-304 (2002).

F.Villani, P.De Maria, E. Ronchi, and M. Galimberti, Oral doxophylline in patients with chronic obstructive pulmonary disease, Int. J. Clin. Pharmacol. Ther., 35, 107(1997).

F.L Dini, L.D Frank, C.Roberto, Doxofylline:A New Generation Xanthine Bronchodilator Devoid of Major Cardiovascular Adverse effects, Curr. Med. Res. Opinion.16, 258-268(2000).

H.R Joshi, A.H Patel, A.D Captain, Spectrophotometric and reversed-phased high performance liquid chromatographic method for the determination of doxofylline in pharmaceutical formulations, J. Young Pharm., 2, 289-296(2010).

F.Tagliaro, R. Dorizzi, A.Fregario, and M.Marigo, Non-extraction HPLC method for simultaneous measurement of dyphylline and doxofylline in serum Clin. Chem., 36,113-5(1990).

M.B.Lagana, A.Marino, M. Mancini, Solid Phase Extraction and high performance liquid chromatographic determination of doxophylline in Plasma, Biomed. Chromatogr. 4, 205-207(1990).

M.Ashu, P. Shikha, Development and Validation of Rapid HPLC Method for determination of doxofylline in Bulk Drug and Pharmaceutical Dosage forms, J. Anal. Chem., 65, 293-297(2010).

B.Umadevi, T. Vetrichelvan, Development And Validation Of UV Spectrophotometric Determination Of Doxofylline And Ambroxol Hydrochloride In Bulk And Combined Tablet Formulation, International Journal of Current Pharmaceutical Research, 3, 89-92(2011).

G.P Narendra, L. Sathiyanarayanan, V.M Mahadeo, R.D Sunil, A validated, stability indicating HPTLC method for analysis of doxofylline, J.Planar Chromatogr.-Mod. TLC.22, 345-348(2009).

R.Gannu, S.Bandari, S.G Sudke, Y. M.Rao, B.P.Shankar, Development and validation Of a stability-indicating RP-HPLC method for analysis of doxofylline In human serum. Application of the method to a pharmacokinetic study, Acta. Chromat.19, 149-160(2007).

N. Sreenivas, M.L.Narasu, B.P Shankar, R. Mullangi, Development and validation of a sensitive LC-MS/MS method with electrospray ionization for quantitation of doxofylline in human serum: application to a clinical pharmacokinetic study, Biomed. Chromatgr. 22, 654-661(2008).

R. Jain, V. K. Gupta, N. Jadon, K. Radhapyari, Voltammetric determination of cefixime in pharmaceuticals and biological fluids., Anal. Biochem. 407, 79-88(2010).

R. Jain, V. K. Gupta, N. Jadon, K. Radhapyari, Adsorptive stripping voltammetric determination of pyridostigmine bromide in bulk, pharmaceutical formulations and biological fluid, J. Electroanal. Chem. 648, 20-27(2010).

R. Jain, R.K. Yadav, Voltmmetric assay of anti-anginal drug nicorandil in different solvents, Drug Testing and Analysis, 3, 171–175(2011).

R. Jain, J.A. Rather; Voltammetric determination of antibacterial drug gemifloxacin in solubilized systems at multi-walled carbon nanotubes modified glassy carbon electrodes, Colloids and Surfaces B: Biointerfaces, 83, 340–346(2011).

R. Jain, J.A. Rather; Stripping voltammetry of tinidazole in solubilized system and biological fluids, Colloids and surfaces A: Physicochem. Eng. Aspects, 378, 27–33(2011).

R. Jain, R. K. Yadav, A. Dwivedi, Square-wave adsorptive stripping voltammetric behavior of entacapone at HMDE and its determination in the presence of surfactants, Colloids Surf. A: Physicochem. Eng. Aspects 359, 25-30(2010).

M. Panizza and G. Cerisola, Application of Diamond electrodes to electrochemical processes, Electrochim. Acta, 51, 191(2005).

M.A.Q. Alfaro, S. Ferro, C.A. Martínez-Huitle and Y.M. Vong, Boron doped Diamond electrode for Waste water Treatment, J. Braz. Chem. Soc., 17, 227(2006).

W. Haenni, P. Rychen, M. Fryda and C. Comninellis, in: Thin-Film Diamond Part B, Ch.Nebel, Editor, Academic Press, Semiconductors and Semimetals series, Elsevier, p. 149 (2004).

A. Kraft, Doped Diamond: A Compact review on new, versatile Electrode Material, Int. J. Electrochem. Sci., 2, 355 – 385(2007).

R. S. Nicholson,I. Shain, Theory of Stationary electrode polarography: single scan and cyclic methods applied to reversible, irreversible and kinetic systems, Anal. Chem., 36, 706(1964).

A.J. Bard, L.R. Faulkner, Electrochemical methods Fundamentals and applications, Wiley, New York, 1980.

DOI: http://dx.doi.org/10.20450/mjcce.2012.42


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