Evaluation of a capillary electrophoresis method for routine determination of varenicline tartrate in quality control laboratories

Authors

  • Mustafa Celebier Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry Ankara- TURKEY
  • Engin Koçak Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry Ankara- TURKEY
  • Sacide Altınöz Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry Ankara- TURKEY

DOI:

https://doi.org/10.20450/mjcce.2015.508

Keywords:

Capillary electrophoresis, varenicline tartrate, analytical method validation, pharmaceutical dosage forms, quality control

Abstract

In this study, analyses were carried out in a fused-silica capillary (i.d. 50.0 µm, total length 48.5 cm and effective length 40.0 cm), in normal mode, applying a voltage of 20 kV. Sample injections were made in a hydrodynamic mode over 7 seconds under a pressure of 50 mbar. Capillary temperature was set at 35 °C and the detection was performed at 205 nm wavelenght. Background electrolyte was 40 mM citrate buffer at pH 6.0 and the internal standard was labetalol HCl. Total analysis time was shorter than 5 minutes. The method was validated according to the ICH guidelines and it was found to be linear, precise, accurate, specific, robust and rugged. Linearity range was found to be 1.0 – 60.0 µg mL-1 and the limit of detection and quantitation were found as 0.5 and 1.0 µg mL-1, respectively. 

 

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Published

2015-11-12

How to Cite

Celebier, M., Koçak, E., & Altınöz, S. (2015). Evaluation of a capillary electrophoresis method for routine determination of varenicline tartrate in quality control laboratories. Macedonian Journal of Chemistry and Chemical Engineering, 34(2), 267–275. https://doi.org/10.20450/mjcce.2015.508

Issue

Vol. 34 No. 2 (2015)

Section

Analytical Chemistry

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