Physical and chemical parameters impact on eosin spectral determinations

Liljana Kola

Abstract


The fluorescence ability of Eosin enables its using as an artificial tracer in the water system studies. The problem deals with the application of Eosin to trace and determine water movements within the karstic system and under ground waters. The fluorescence intensity of fluorescent dyes in water samples depends on their physical and chemical properties, such as pH, temperature, presence of oxidants, etc. Besides that, the UV radiation may induce photochemical decomposition of Eosin which can cause large measurement errors. This paper presents the taken results studying the influence of these factors on Eosin fluorescence intensity using the concentration and synchron scan methods. The method we have elaborated for this purpose made it possible to optimize procedures we use to analyze water samples for the presence of Eosin and measure its content, even in trace levels by the means of the Perkin Elmer LS 55 Luminescence Spectrometer.


Keywords


spectral determination; Eosin; fluorescence intensity (IF); synchron scan; artificial tracer

Full Text:

PDF

References


. H. Behrens, Tracer Methods in Isotope Hydrology, IAEA, Vienna, 1983.

. H. Behrens, Water Tracer Chemistry – Factor Determining Performance and Analytics of Tracers; 5th Int. Symp. on Underground Water Tracing, Athens, 1986, pp. 121–55 133.

. W. Kass, Tracing Technique in Geohydrology, Berlin,1994.

. R. Benischke, Fluorescent Tracers in Hydrology, Graz, 1991.

. L. Kola, Optimization of Fluorometric Determinations in Water Samples and Active Coal Samples (Albanian Printing), Ph.D. Thesis, Tirana University, 2008.

. Perkin Elmer, Molecular Spectroscopy, LS 55 Users Guide, Buckinghamshire, UK, 2000.

. Perkin Elmer, Fluorescence Intensity Samples, C520-7440, Booklet, 2000.




DOI: http://dx.doi.org/10.20450/mjcce.2010.172

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Liljana Kola

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.