Physical and chemical parameters impact on eosin spectral determinations

Liljana Kola


The fluorescence ability of Eosin enables its using as an artificial tracer in the water system studies. The problem deals with the application of Eosin to trace and determine water movements within the karstic system and under ground waters. The fluorescence intensity of fluorescent dyes in water samples depends on their physical and chemical properties, such as pH, temperature, presence of oxidants, etc. Besides that, the UV radiation may induce photochemical decomposition of Eosin which can cause large measurement errors. This paper presents the taken results studying the influence of these factors on Eosin fluorescence intensity using the concentration and synchron scan methods. The method we have elaborated for this purpose made it possible to optimize procedures we use to analyze water samples for the presence of Eosin and measure its content, even in trace levels by the means of the Perkin Elmer LS 55 Luminescence Spectrometer.


spectral determination; Eosin; fluorescence intensity (IF); synchron scan; artificial tracer

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