The influence of some chemical and physical parameters of water samples on spectral determinations of fluorescent dyes

Authors

  • Liljana Kola Department of Technology, Institute of Nuclear Physics, Alexander Moisiu Street, P.O. Box 85, Tirana,
  • Sokrat Amataj Department of Technology, Institute of Nuclear Physics, Alexander Moisiu Street, P.O. Box 85, Tirana,

DOI:

https://doi.org/10.20450/mjcce.2006.293

Keywords:

spectrofluorometer, fluorescence, artificial tracer, spectrum, synchronscan, uranine, rhodamine WT, sulphorhodamine G extra

Abstract

The fluorescence intensity of various substances is dependent on the physical and chemical properties (e.g. pH, temperature, sorption, presence of oxidants, etc.) of the respective sample matrix (e.g. water), We have studied the influence of some of these factors on the fluorescence intensity of uranine, rhodamine WT, and sulphorhodamine, in order to enable their detection in trace levels by putting respective aquatic samples in optimal conditions. The results indicate that pH modifies the fluorescence intensity of uranine solutions much more than for the fluorescence of rhodamine WT and sulphorhodamine water solutions. This process is reversible.

Temperature also has influence on the fluorescence intensity, whereas the presence of oxidants in water samples irreversibly destroy the fluorescing molecules. All of these processes manifest diverse effects on different kinds of fluorescent dyes.

References

H. Behrens, Tracer Methods in Isotope Hydrology, IAEA, Vienna, 1983, 173.

S. Amataj, Some Aspects of Nuclear Techniques Application on the Study of Aquatic Media in our Country, Dissertation, Institute of Nuclear Physics, (Aspekte të Përdorimit të Teknikave Bërthamore për Studimin e Mjediseve Ujorë në Vendin Tonë, Disertacion, Instituti i Fizikës Bërthamore), Tirana, 1983.

S. Amataj, T. Anovski, L. Kola, A. Stamos, Studying Underground Communication between Prespa and Ohrid Lakes Through Artificial Tracer Methods (Studimi me Gjurmues Artificial i Komunikimeve Nëntokësore Midis Liqeneve të Ohrit dhe Prespës së Madhe), Tirana, 2004.

Molekular Spektroscopy, LS 55 User’s Guide, Perkin Elmer, Buckingamshire, UK, 2000, 191–196, 283–287.

Fluorescence Intensity Samples, C520-7440, Perkin Elmer (booklet), 10.

W. Käss, Geohydrologische Markierungstechnik, Lehrbuch der Hydrogeologie, Band 9; Gebrüder Borntrager, Berlin, Stuttgart; 1992; 19–25, 34–38, 50–52, 65–68.

R. Benischke, Fluorescent Tracers in Hydrology, Graz, 1991, 12, 22–24, 33.

Downloads

Published

2006-12-15

How to Cite

Kola, L., & Amataj, S. (2006). The influence of some chemical and physical parameters of water samples on spectral determinations of fluorescent dyes. Macedonian Journal of Chemistry and Chemical Engineering, 25(2), 107–112. https://doi.org/10.20450/mjcce.2006.293

Issue

Vol. 25 No. 2 (2006)

Section

Analytical Chemistry

License

The authors agree to the following licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

  • Adapt — remix, transform, and build upon the material
  • for any purpose, even commercially.
The licensor cannot revoke these freedoms as long as you follow the license terms.

 Under the following terms:

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

  • NonCommercial — You may not use the material for commercial purposes.