Impact of oil extraction method and genotype on the techno- and bio-functional properties of Camelina (Camelina sativa L.) seed cake

Authors

DOI:

https://doi.org/10.20450/mjcce.2026.3455

Keywords:

Camelina seed cake, Nutritional compostion, Techno-functional properties, Phenolic profile, Antiradical activity

Abstract

Oilseed cakes are significant by-products of the oil-processing industry with considerable potential for further valorization. This study systematically investigated the effects of genotype (NS Zlatka and NS Slatka) and oil extraction method (Soxhlet extraction with n-hexane versus cold pressing) on the nutritional composition, techno- and bio-functional properties of camelina (Camelina sativa L.) seed cake. Basic nutritional parameters, water and oil absorption capacities, as well as gelling, foaming, and emulsifying properties, were evaluated. The results demonstrated that both genotype and oil extraction method significantly affected the composition and techno-functional properties of camelina seed cake (p < 0.05). Cakes obtained using Soxhlet extraction exhibited higher protein (41.80–42.50 %) and ash (6.11–6.32 %) contents, along with enhanced water (573–699 %) and oil (218–244 %) absorption capacities. In contrast, the resulting cold-pressed cakes retained a higher proportion of residual oil (14.80–16.20%). Although total phenolic content was relatively similar among samples (approximately 0.55 g gallic acid equivalent (GAE)/100 g dry matter), the qualitative and quantitative composition of individual phenolic compounds varied markedly depending on genotype and oil extraction method. The cold-pressed cakes, particularly the NS Zlatka genotype, were characterized by high gallic acid content (140.58 mg/100 g dry matter) and the most pronounced antiradical activity (IC50 = 0.080 mg/ml) determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. These findings indicate that Soxhlet-extracted cakes are superior for applications requiring high protein content and hydration properties, while cold-pressed cakes from the NS Zlatka genotype offer higher antioxidant potential. This study provides a strategic basis for selecting specific genotypes and processing methods to tailor the functional profile of camelina by-products for the food and pharmaceutical industries.

References

REFERENCES

(1) Grahovac, N.; Aleksić, M.; Trajkovska, B.; Marjanović Jeromela, A.; Nakov, G. Extraction and valorization of oilseed cakes for value-added food components—A re-view for a sustainable foodstuff production in a case pro-cess approach. Foods 2025, 14 (13).

DOI: 10.3390/foods14132244.

(2) Singh, B.; Oberoi, S.; Kaur, M.; Kaur, A. Chemical pro-file and bioactive constituents of chia seed cake: A by-product of oil processing industries. Biomass Convers. Biorefin. 2025, 15 (24), 31417–31432.

DOI: 10.1007/s13399-025-06784-2.

(3) Petraru, A.; Amariei, S.; Senila, L. Flaxseed oilcake: An ingredient with high nutritional value in the realization of innovative food products. Foods 2025, 14 (7).

DOI: 10.3390/foods14071087.

(4) Hamza, H.; Kaushik, D.; Kaur, H.; Kaur, R.; Bordiga, M.; Jaouhari, Y.; Proestos, C.; Ahmed, M.; Khan, M. R.; Oz, F.; et al. Optimizing and characterization of soybean oil seed cake protein hydrolysis: In vitro analysis. Food Sci. Nutr. 2025, 13 (5). DOI: 10.1002/fsn3.70270.

(5) Souza, M. A. d. A.; Rudke, A. R.; Germano, A. T.; Vi-tali, L.; Ferreira, S. R. S. Sequential high-pressure extrac-tion using green solvents to recover bioactive compounds from sesame cake. J. Supercrit. Fluids 2025, 215. DOI: 10.1016/j.supflu.2024.106421.

(6) Saorin Puton, B. M.; Demaman Oro, C. E.; Lisboa Ber-nardi, J.; Exenberger Finkler, D.; Venquiaruto, L. D.; Dallago, R. M.; Tres, M. V. Sustainable valorization of plant residues through enzymatic hydrolysis for the ex-traction of bioactive compounds: Applications as func-tional ingredients in cosmetics. Processes 2025, 13 (5). DOI: 10.3390/pr13051314.

(7) Rakita, S.; Kokić, B.; Manoni, M.; Mazzoleni, S.; Lin, P.; Luciano, A.; Ottoboni, M.; Cheli, F.; Pinotti, L. Cold-pressed oilseed cakes as alternative and sustainable feed ingredients: A review. Foods 2023, 12 (3).

DOI: 10.3390/foods12030432.

(8) Salam, S.; Inoussa, C.; Patrice, B.; Firmin, S.; Fréderic, B. S.; Mipro, H. Nutritional value and antinutritional fac-tors of Balanites aegyptiaca seed oils and cakes for ani-mal feed: A review. Food Sci. Nutr. 2025, 13 (6). DOI: 10.1002/fsn3.70478.

(9) Lotfi, M.; Keshvari, T.; Taghizadeh, M. S.; Afsharifar, A.; Moghadam, A.; Aram, F.; Niazi, A. Optimizing Camelina sativa oil extraction and its cytotoxicity using RSM by emphasis on antioxidant properties, physical characteristics, and molecular docking insights. Ind. Crops Prod. 2025, 224.

DOI: 10.1016/j.indcrop.2024.120334.

(10) Rakita, S.; Spasevski, N.; Savić, I.; Savić Gajić, I.; Laz-arević, J.; Dragojlović, D.; Đuragić, O. Comparative evaluation of camelina seed oils obtained by cold-pressing and solvent extraction. Foods 2024, 13 (22). DOI: 10.3390/foods13223605.

(11) Angelopoulou, F.; Roussis, I.; Kakabouki, I.; Mavroeidis, A.; Triantafyllidis, V.; Beslemes, D.; Kosma, C.; Stav-ropoulos, P.; Tsiplakou, E.; Bilalis, D. Influence of or-ganic fertilization and soil tillage on the yield and quality of cold-pressed camelina [Camelina sativa (L.) Crantz] seed cake: An alternative feed ingredient. Appl. Sci. 2023, 13 (6). DOI: 10.3390/app13063759.

(12) Rakita, S.; Spasevski, N.; Vidosavljević, S.; Tomičić, Z.; Savić, I. M.; Savić Gajić, I. M.; Đuragić, O.; Marjanović Jeromela, A. Valorization of camelina cake by fractiona-tion: Characterization of nutritional and functional proper-ties. Foods 2025, 14 (19).

DOI: 10.3390/foods14193437.

(13) Piergiovanni, A. R.; Alberghini, B.; Zanetti, F.; Ponzoni, E.; Brambilla, I. M.; Galasso, I. The effect of year and va-riety on the nutritional value of Camelina sativa meal. Eur. Food Res. Technol. 2025, 251 (5), 841–849.

DOI: 10.1007/s00217-025-04672-7.

(14) Ilić, P.; Rakita, S.; Spasevski, N.; Đuragić, O.; Marjanov-ić-Jeromela, A.; Cvejić, S.; Zanetti, F. Nutritive value of Serbian camelina genotypes as an alternative feed ingredi-ent. Food and Feed Research 2022, 49 (2), 209–221. DOI: 10.5937/ffr49-41060.

(15) Šuput, D.; Lončar, B.; Popović, S.; Hromiš, N.; Rakita, S.; Spasevski, N. The influence of the type and concentra-tion of plasticizer on the properties of biopolymer films based on wild flax (Camelina sativa). Anal. Tech. Szeged. 2024, 18 (3), 69–76.

DOI: 10.14232/analecta.2024.3.69-76.

(16) ISO. Animal feeding stuffs – Determination of moisture and other volatile matter content; ISO 6496:1999; Gene-va, Switzerland, 1999.

(17) ISO. Animal feeding stuffs – Determination of nitrogen content and calculation of crude protein content – Part 1: Kjeldahl method; ISO 5983-1:2010; Geneva, Switzer-land, 2005.

(18) ISO. Animal feeding stuffs – Determination of fat content; ISO 6492:1999; Geneva, Switzerland, 1999.

(19) ISO. Animal feeding stuffs – Determination of crude ash; ISO 5984:2022; Geneva, Switzerland, 2022.

(20) AOCS. Crude fiber analysis in feeds by filter bag tech-nique; AOCS Approved Procedure Ba 6a-05; Cham-paign, IL, USA, 2005.

(21) AOAC. Official methods of analysis of AOAC INTER-NATIONAL; Official Method 973.40 (pH of Animal Feed); Association of Official Agricultural Chemists, 2023.

(22) Sosulski, F. The centrifuge method for determining flour absorption in hard red spring wheats. Cereal Chem. 1962, 39, 344–350.

(23) Lin, M. J.-Y.; Humbert, E.; Sosulski, F. Certain function-al properties of sunflower meal products. J. Food Sci. 1974, 39 (2), 368–370.

DOI: 10.1111/j.1365-2621.1974.tb02896.x.

(24) Coffmann, C.; Garciaj, V. Functional properties and ami-no acid content of a protein isolate from mung bean flour. Int. J. Food Sci. Tech. 1977, 12 (5), 473–484. DOI: 10.1111/j.1365-2621.1977.tb00132.x.

(25) Lawhon, J. T.; Cater, C. M.; Mattil, K. F. A comparative study of the whipping potential of an extract from several oilseed flours. Cereal Sci Today 1972, 17 (9), 240–246.

(26) Yasumatsu, K.; Sawada, K.; Moritaka, S.; Misaki, M.; Toda, J.; Wada, T.; Ishii, K. Whipping and emulsifying properties of soybean products. Agric. Biol. Chem. 1972, 36 (5), 719–727.

DOI: 10.1080/00021369.1972.10860321.

(27) Savic Gajic, I.; Savic, I.; Boskov, I.; Žerajić, S.; Mar-kovic, I.; Gajic, D. Optimization of ultrasound-assisted extraction of phenolic compounds from black locust (Ro-biniae pseudoacaciae) flowers and comparison with con-ventional methods. Antioxidants 2019, 8 (8).

DOI: 10.3390/antiox8080248.

(28) Nour, V.; Trandafir, I.; Cosmulescu, S. HPLC determina-tion of phenolic acids, flavonoids and juglone in walnut leaves. J. Chromatogr. Sci. 2013, 51 (9), 883–890. DOI: 10.1093/chromsci/bms180.

(29) Abdullah, M.; Ch’ng, P.; Lim, T. Some physical proper-ties of Parkia speciosa seeds. In International Confer-ence on Food Engineering and Biotechnology, 2011; Vol. 9, pp 43–47.

(30) Riaz, R.; Ahmed, I.; Sizmaz, O.; Ahsan, U. Use of Camelina sativa and by-products in diets for dairy cows: A review. Animals 2022, 12 (9).

DOI: 10.3390/ani12091082.

(31) Tienda-Vazquez, M. A.; Perez-Herrera, M.; Carrasco-Morales, O.; Tellez-Perez, C.; Alonzo-Macias, M.; Car-dador-Martínez, A. Techno-functional properties of Camelina sativa cake proteins treated with the instant controlled pressure drop (DIC) technology. Innov. Food Sci. Emerg. Technol. 2024, 95.

DOI: 10.1016/j.ifset.2024.103741.

(32) Delver, J. J.; Smith, Z. K. Opportunities for camelina meal as a livestock feed ingredient. Agriculture 2024, 14 (1). DOI: 10.3390/agriculture14010116.

(33) Rebbah, K.; Meziani, S.; Demmouche, A.; Labga, L.; Amara, L.; Badri, F. Z.; Ghaffari, I.; Chenni, F. Z.; Tou-mi, M. N.; Menadi, N. Nutritional and antioxidant profil-ing of Algerian rapeseed seeds (Brassica napus L.) and its cold-pressed by-products. Croat. J. Food Sci. Technol. 2025, 17 (1), 81–100.

DOI: 10.17508/cjfst.2025.17.1.06.

(34) Yalçın, S.; Ramay, M. S.; Yalçınkaya, H.; Kardoğan, Ö.; Erkurt, A.; Kılınç, B.; Gebeş, E. S.; Bundur, A.; On-başılar, E. E.; Yalçın, S.; et al. Evaluation of high levels of solvent extracted Camelina sativa meal in diets on per-formance, blood indices, cecal microorganisms, and nutri-ent digestibility in broilers. Trop. Anim. Health Prod. 2025, 57 (2). DOI: 10.1007/s11250-025-04339-1.

(35) Mollaei, S.; Hosseini-Nami, S. M.; Hazrati, S. Evaluation of the nutritional value and active compounds of commer-cial and non-commercial oilseed meals. Sci. Rep. 2025, 15 (1). DOI: 10.1038/s41598-025-10671-1.

(36) Lolli, S.; Grilli, G.; Ferrari, L.; Battelli, G.; Pozzo, S.; Galasso, I.; Russo, R.; Brasca, M.; Reggiani, R.; Fer-rante, V. Effect of different percentage of Camelina sativa cake in laying hens diet: Performance, welfare, and egg-shell quality. Animals 2020, 10 (8).

DOI: 10.3390/ani10081396.

(37) Chuang, W.-Y.; Lin, L.-J.; Shih, H.-D.; Shy, Y.-M.; Chang, S.-C.; Lee, T.-T. The potential utilization of high-fiber agricultural by-products as monogastric animal feed and feed additives: A review. Animals 2021, 11 (7). DOI: 10.3390/ani11072098.

(38) El-Beltagi, H. S.; Ahmed, A. R.; Mohamed, H. I.; Al-Otaibi, H. H.; Ramadan, K. M. A.; Elkatry, H. O. Utiliza-tion of prickly pear peels flour as a natural source of min-erals, dietary fiber and antioxidants: Effect on cakes pro-duction. Agronomy 2023, 13 (2).

DOI: 10.3390/agronomy13020439.

(39) Olukomaiya, O. O.; Fernando, W. C.; Mereddy, R.; Li, X.; Sultanbawa, Y. Physicochemical, microbiological and functional properties of camelina meal fermented in solid-state using food grade Aspergillus fungi. Fermentation 2020, 6 (2). DOI: 10.3390/fermentation6020044.

(40) Saju, F.; Sivaraman, C. M. Scope of herbal mucilage in pharmaceutical formulations. A review. Herba Pol. 2021, 67 (1), 46–57. DOI: 10.2478/hepo-2021-0001.

(41) Kakar, A.; Miano, T. F.; Soomro, A. H.; Yar, A.; Memon, S. A.; Khan, B. Oil and water absorption capaci-ty of wheat, rice and gram flour powders. Int. J. Ecosyst. Ecol. Sci. 2022, 12 (2), 585–594.

DOI: 10.31407/ijees.

(42) Zhang, Y.; Sharan, S.; Rinnan, Å.; Orlien, V. Survey on methods for investigating protein functionality and related molecular characteristics. Foods 2021, 10 (11).

DOI: 10.3390/foods10112848.

(43) Drozłowska, E.; Starowicz, M.; Śmietana, N.; Krupa-Kozak, U.; Łopusiewicz, Ł. Spray-drying impact the physicochemical properties and formation of Maillard re-action products contributing to antioxidant activity of camelina press cake extract. Antioxidants 2023, 12 (4). DOI: 10.3390/antiox12040919.

(44) Kramar, A.; Laguta, I.; Anishchenko, V.; Stavinskaya, O.; Kuzema, P.; Smirnova, N.; Ivannikov, R.; Linnik, O. Comparative study on phenolic content and antioxi-dant/reducing properties of extracts from various types of camelina plant biomass. Waste Biomass Valoriz. 2025, 16 (9), 4553–4566.

DOI: 10.1007/s12649-025-02931-y.

(45) Rodríguez-Castillo, P.; Saab-Mejía, H.; Batallas-Tituaña, I.; Alonzo-Macías, M.; Téllez-Pérez, C.; Cardador-Martínez, A. "Impact of instant controlled pressure drop (DIC) technology on the antioxidant properties of Came-lina sativa cake". Food Chem. Adv. 2025, 9. DOI: 10.1016/j.focha.2025.101195.

(46) Tavarini, S.; De Leo, M.; Matteo, R.; Lazzeri, L.; Braca, A.; Angelini, L. G. Flaxseed and camelina meals as po-tential sources of health-beneficial compounds. Plants 2021, 10 (1). DOI: 10.3390/plants10010156.

(47) Xiang, Z.; Guan, H.; Zhao, X.; Xie, Q.; Xie, Z.; Cai, F.; Dang, R.; Li, M.; Wang, C. Dietary gallic acid as an anti-oxidant: A review of its food industry applications, health benefits, bioavailability, nano-delivery systems, and drug interactions. Food Res. Int. 2024, 180.

DOI: 10.1016/j.foodres.2024.114068.

Downloads

Published

2026-06-26

How to Cite

Savić, I., Savic Gajic, I., Rakita, S., Spasevski, N., & Bajić, A. (2026). Impact of oil extraction method and genotype on the techno- and bio-functional properties of Camelina (Camelina sativa L.) seed cake. Macedonian Journal of Chemistry and Chemical Engineering, 45(1). https://doi.org/10.20450/mjcce.2026.3455

Issue

Section

Natural Products