RP-HPLC-DAD method for simultaneous determination of desmedipham, phenmedipham and ethofumesate in a pesticide formulation

Authors

  • Lenče Velkoska-Markovska Faculty of Agricultural Sciences and Food, Ss. Cyril and Methodius University, Blvd. Aleksandar Makedonski bb, P.O. Box 297, 1000 Skopje
  • Biljana Petanovska-Ilievska Faculty of Agricultural Sciences and Food, Ss. Cyril and Methodius University, Blvd. Aleksandar Makedonski bb, P.O. Box 297, 1000 Skopje
  • Lila Vodeb Faculty of Agricultural Sciences and Food, Ss. Cyril and Methodius University, Blvd. Aleksandar Makedonski bb, P.O. Box 297, 1000 Skopje

DOI:

https://doi.org/10.20450/mjcce.2012.55

Keywords:

desmedipham, phenmedipham, ethofumesate, RP-HPLC, UV-DAD

Abstract

A fast, simple, precise and accurate reversed-phase high-performance liquid chromatography (RPHPLC)method with UV-DAD for simultaneous determination of desmedipham, phenmedipham and ethofumesate in the pesticide formulation “Inter OF” has been developed. The analysis was performed on a LiChrospher 60 RP-select B (25 cm × 0.4 cm, 5 μm, Merck) analytical column, with mobile phase of methanol/water (60/40, V/V), flow rate of 1 ml/min, UV-detection at 230 nm and constant column temperature at 25 ºC. The following parameters were determined for the developed method: retention factor, separation factor, limit of detection (LOD), limit of quantification (LOQ), precision of obtained results for peak area, linearity, recovery of analyte and active ingredients quantity in a pesticide formulation.

References

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Published

2012-06-15

How to Cite

Velkoska-Markovska, L., Petanovska-Ilievska, B., & Vodeb, L. (2012). RP-HPLC-DAD method for simultaneous determination of desmedipham, phenmedipham and ethofumesate in a pesticide formulation. Macedonian Journal of Chemistry and Chemical Engineering, 31(1), 39–46. https://doi.org/10.20450/mjcce.2012.55

Issue

Vol. 31 No. 1 (2012)

Section

Analytical Chemistry

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