Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications

Nevena Jaćimović; Marija  Djošić; Ana Jankovic; Svetlana  Grujić; Ivana  Matić Bujagić; Jovica  Stojanović; Maja  Vukašinović-Sekulić; Vesna  Kojić; Vesna  Mišković-Stanković

doi:10.20450/mjcce.2023.2775

Authors

Nevena Jaćimović Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia
Marija Djošić Institute for Technology of Nuclear and Other Mineral Raw Materials, Bulevar Franš d'Eperea 86, Belgrade, Serbia https://orcid.org/0000-0002-0869-2182
Ana Jankovic Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia https://orcid.org/0000-0002-5605-3297
Svetlana Grujić Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia https://orcid.org/0000-0003-4787-1391
Ivana Matić Bujagić Academy of Applied Technical Studies Belgrade, Katarine Ambrozić 3, Belgrade, Serbia https://orcid.org/0000-0001-8459-873X
Jovica Stojanović Institute for Technology of Nuclear and Other Mineral Raw Materials, Bulevar Franš d'Eperea 86, Belgrade, Serbia https://orcid.org/0000-0002-2752-6374
Maja Vukašinović-Sekulić Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia https://orcid.org/0000-0001-5374-5020
Vesna Kojić Oncology Institute of Vojvodina, University of Novi Sad, Put Dr Goldmana 4, Sremska Kamenica, Serbia https://orcid.org/0000-0002-2399-0807
Vesna Mišković-Stanković Faculty of Ecology and Environmental Protection, University Union - Nikola Tesla, Cara Dušana 62-64, Belgrade, Serbia https://orcid.org/0000-0001-6525-9820

DOI:

https://doi.org/10.20450/mjcce.2023.2775

Keywords:

electrophoretic deposition, Chitosan, poly(vinyl alcohol),, gentamicin release, antibacterial

Abstract

Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron microscopy with field emission (FE–SEM), Fourier transform infrared spectroscopy (FT–IR) and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using a high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30% of gentamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. Antibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherichia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high applicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.

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Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications

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