Zinc complex based determination of rutin in dietary supplements


  • Leposava Pavun University of Belgrade-Faculty of Pharmacy
  • Milena Jelikić Stankov University of Belgrade-Faculty of Pharmacy
  • Predrag Đurđević Faculty of Science, University of Kragujevac
  • Andrija Ćirić Faculty of Science, University of Kragujevac
  • Snezana Uskokovic-Markovic University of Belgrade-Faculty of Pharmacy




flavonoids, rutin, zinc complex, spectrophotometric determination


The aim of this study was to develop and validate a simple, rapid and sensitive and low-cost method for determination of rutin in tablets. The proposed spectrophotometric method is based on the formation of the Zn2+-rutin complex in 70 v/v% methanol at pH 8.52, and λmax= 410 nm. Linearity range was defined as 0.3- 12.2 mg mL-1, LOD = 0.21 mg mL-1 and LOQ = 0.63 mg mL-1. The proposed method was successfully applied for determination of rutin in herbal based dietary supplements. The reliability of the method was checked by comparison with results obtained by RP-HPLC/UV method. The proposed method fulfills all aimed requirements.

Author Biography

Snezana Uskokovic-Markovic, University of Belgrade-Faculty of Pharmacy

Department of Analytical Chemistry


M.L. Falcone Ferreyra, S.P. Rius, P. Casati, Flavonoids: biosynthesis, biological functions, and biotechnological applications, Front Plant Sci. 28, (2012). Sep 222.

F. Galeotti, E. Barile, P. Curir, M. Dolci, V. Lanzotti, Flavonoids from carnation (Dianthus caryophyllus) and their antifungal activity. Phytochem. Lett. 1, 44 (2008).

O.M. Andersen, K.R. Markham, Flavonoids: Chemistry, biochemistry and applications. Boca Raton, FL: CRC/Taylor & Francis; 2006.

R.J. Nijveldt, E. van Nood, D.E. van Hoorn, P.G. Boelens, K. van Norren, P.A. van

Leeuwen, Flavonoids: a review of probable mechanisms of action and potential applications, Am. J. Clin. Nutr. 74, 418–425 (2001).

L. Le Marchand, Cancer preventive effects of flavonoids:a review, Biomed.Pharmacother. 56, 296–303 (2002).

I.B. Afanas’ev, E.A. Ostrakhovitch, E.V. Mikhal’chik, G.A. Ibragimova, L.G. Korkina, Enhancement of antioxidant and anti-inflammatory activities of bioflavonoid rutin by complexation with transition metals, Biochem. Pharmacol. 61, 677–684 (2001).

M.M. Bratu, S. Birghila, H. Miresan, T. Negreanu-Pirjol, C. Prajitura, M. Calinescu, Biological activities of Zn(II) and Cu(II) complexes with quercetin and rutin: Antioxidant properties and UV-protection capacity, Rev. Chim. (Bucharest) 65, 544-549 (2014).

N.E.A. Ikeda, E. M. Novak, D. A. Maria, A. S. Velosa, R. M. S. Pereira, Synthesis, characterization and biological evaluation of rutin–zinc(II) flavonoid–metal complex, Chem. Bio. Interact. 239, 184-191 (2015).

Y. Bai, F. Song, M. Chen, J. Xing, Z. Liu, S. Liu S Characterization of the rutin-metal complex by electrospray ionization tandem mass spectrometry, Anal. Sci. 2, 1147-1151 (2004).

F. Shahidi, C.T. Ho, Phytochemicals and Phytopharmaceuticals AOCS Press: Champaign, USA, 1999.

V.A. Kostyuk, A.I. Potapovich, E.N. Strigunova, T.V. Kostyuk, I.B. Afanas’ev, Experimental evidence that flavonoid metal complexes may act as mimics of superoxide dismutase, Arch. Biochem. Biophys. 428, 204–208 (2004).

J. Yoe, A. Jones, Ind. Eng. Chem. Anal. Ed. 16: 111. (1944).

H. Irving, T. Pierce, J. Chem. Soc. 2565 (1959).

V. Kuntić, D. Malešev, Z. Radović, M. Kosanić, U. Mioč, V. Vukojević, Spectrophotometric Investigation of Uranil(II)−Rutin Complex in 70 Ethanol, J. Agric. Food Chem., 46, 5139–5142 (1998).

Specifications and Control Tests on the Finished Product. Directive 75/318/EEC as amended (3AQ11a)




How to Cite

Pavun, L., Jelikić Stankov, M., Đurđević, P., Ćirić, A., & Uskokovic-Markovic, S. (2016). Zinc complex based determination of rutin in dietary supplements. Macedonian Journal of Chemistry and Chemical Engineering, 35(1), 13–18. https://doi.org/10.20450/mjcce.2016.897



Analytical Chemistry

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