Simultaneous RP-HPLC-DAD determination of dansyl amino acids in chemically treated human hair

Authors

  • Pece Sherovski Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje
  • Marina Stefova Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje
  • Natasha Ristovska Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje

DOI:

https://doi.org/10.20450/mjcce.2018.1594

Keywords:

HPLC, dansyl amino acids, protein hydrolisate, hair proteins, bleaching, protein treatments,

Abstract

A simple, rapid and reliable RP-HPLC-DAD method for the analysis of 19 amino acids in the protein hydrolysate of chemically treated hair is developed. The pre-column dansylation and RP-HPLC separation were optimized and peak resolution was improved as compared to previously published HPLC methods. The developed method showed excellent linearity for all tested amino acids in the concentration range 0.05-0.5 mmol/l. The obtained results show that the concentration of all amino acids detected in bleached hair decreases with increasing the concentration of hydrogen peroxide, this is most significant for cystine, compared to virgin hair. Higher amounts of the typical amino acids for keratin have been measured after exposure of hair to protein treatments. The method has been shown as very useful for amino acids analysis in a protein hydrolisate and it can be also used for establishing the amino acid profiles of other protein samples using this optimized procedure.

References

V. N. E. Robinson, A study of damaged hair, J. Soc.Cosmet.Chem.27, 155-161 (1976).

C. R. Robbins, Chemical and Physical Behavior of Human Hair, SPRINGER - Ed. Verlag, 5th ed. (2012). DOI: 10.1007/978-3-642-25611-0

C. Tomes, J. T. Jones, C. M. Carr, D. Jones, Three-dimensional imaging and analysis of the surface of hair fibers using scanning electron microscopy. Int. J. Cosmet. Sci. 29, 293–299 (2007).

R. De Cassia, C. S. Wagner, P. K. Kiyohara, M. Silveira, I. Joekes, Electron microscopic observations of human hair medulla, J. Microsc. 226, 54–63 (2007).

J. A. Swift, J. R. Smith, Atomic force microscopy of human hair, Scanning. 22, 310-318 (2000).

S .B. Ruetsch, Y. K. Kamath, L. Kintrup, H-J. Schwark, Effects of conditioners on surface hardness of hair fibers: An investigation using atomic force microscopy, J. Cosmet. Sci. 54, 579-588 (2003).

M. V. R. Velasco, A. R. Baby, F. D. Sarruf, T. M. Kaneko, R. E. Samad, N. D. Vieira Júnior, A. Z. de Freitas, Prospective ultramorphological characterization of human hair by optical coherence tomography, Skin. Res. Technol. 15(4), 440–443 (2009).

C. Bouillon, J. Wilkinson, The Science of Hair Care, 2nd ed. CRC Press: Boca Raton, FL, USA, (2005).

J-L Bantignies, G. L. Carr, D. Lutz, S. Marull, G. P. Williams, G. Fuchs, Chemical imaging of hair by infrared microspectroscopy using synchrotron radiation, J.Cosmet.Sci., 51, 73-90 (2000).

A. Kuzuhara, Analysis of structural change in keratin fibers resulting from chemical treatments using Raman spectroscopy. Biopolymers. 77(6), 335–344 (2005). doi:10.1002/bip.20221.

B. C. Beard, J. Hare, Surface interaction of quaternary amines with hair, J. Surfactants Deterg. 5(2), 145-150 (2002).

N. Nishikawa, Y. Horiguchi, T. Asakura, I. Ando,

Carbon-13 solid-state nmr study of 13C-enriched human hair keratin, Polymer. 40, 2139–2144 (1999).

S. S. Sandhu, C. Robbins, A simple and sensitive technique, based on protein loss measurements, to assess surface damage to human hair, J. Soc. Cosmet. hem. 4, 163-175 (1993).

A. L. Silva, A. S. Nunes, J. L. Gesztesi, Protein loss quantification of abraded virgin and abraded bleached hair according to Bradford assay. J Cosmet Sci. 55, 175-179 (2004).

P. Sherovski, G. Stojković, N. Ristovska, Development, validation and application of first derivative spectroscopy ratio method for estimation of Bradford assay, Anal. Biochem. 558, 35–40 (2018).

S. A. França-Stefoni, M. F. Dario, T. C. Sá-Dias, V. Bedin, A. J. Almeida, A. R. Baby, M. V. Velasco, Protein loss in human hair from combination straightening and coloring treatments. J. Cosmet. Dermatol. 14, 204–208 (2015).

S. B. Ruetsch, B. Yang, Y. K. Kamath, Chemical and photo-oxidative hair damage studied by dye diffusion and electrophoresis. J. Cosmet. Sci. 54, 379–394 (2003).

P. Sherovski, N. Ristovska, Electrophoretic analysis of proteins of chemical treated human hair, IJOER. 4(5), 38-42 (2018).

C. F. Cruz, C. Costa, A. C. Gomes, T. Matamá, A. Cavaco-Paulo, Human hair and the impact of cosmetic procedures: A Review on cleansing and shape-modulating cosmetics, Cosmetics. 3, 26 (2016).

I. Molnftr-Perl, Quantitation of amino acids and amines by chromatography, J. Chromatogr. Libr. 70 (9) 2005.

A. Fabiani, A. Versari, G. P. Parpinello, M. Castellari, S. Galassi, High-performance liquid chromatographic analysis of free amino acids in fruit juices using derivatization with 9-fluorenylmethyl-chloroformate, Journal of Chromatografic Science. 40, 14–18 (2002).

T. Takeuchi, HPLC of amino acids as dansyl and dabsyl derivatives, J. Chromatogr. Libr. 70, 229–241 (2005).

P. Molnar, New aspects of the simultaneous analysis of amino acids and amines as their o-phthaldialdehyde derivatives by high-performance liquid chromatography analysis of wine, beer and vinegar, J. Chromatogr A. 987, 311-22 (2003).

H. Wang,Y. Zhao, H. Jin, H. Zhang, N-Hydroxysuccinimidyl-α naphthylacetate as a derivatizing reagent for amino acids and oligopeptides in PR-HPLC, J.Liq.Chrom. & Rel.Tech. 24(20), 3157–3170 (2001)

R. Minocha, S. Long, Simultaneous separation and quantitation of amino acids and polyamines of forest tree tissues and cell cultures within a single high-performance liquid chromatography run using dansyl derivatization, J.Chromatography A. 1035, 63-73 (2004).

C. Rutledge, J. Rudy, HPLC qualitative amino acid analysis in the clinical laboratories, A.J.C.P. 5, 614-618 (1987).

Y. Tapuhi, D. E. Schmidt, W. Lindner, B. L. Karger, Dansylation of amino acids for high-performance liquid chromatography analysis. Anal. Biochm. 115(1), 123–129 (1981). doi:10.1016/0003-2697(81)90534-0

M. Fountoulakis, H. Lahm, Hydrolysis and amino acid composition analysis of proteins J.Chromatog. A. 826(2), 109-134 (1998).

J. M. Wilkinson, CRC Handbook of HPLC for the separation of amino acids, peptides, and proteins, CRC Ress, Boca Raton, 1984

K. Tašev, V. Ivanova-Petropulos, M. Stefova, Optimization ana validation od derivatization method for analysis of biogenic amines in wines using RP-HPLC-DAD, Maced. J. Chem. Chem. Eng. 35, 19–28 (2016).

R. Sethuraman, T. L. Lee, S. Tachiban, Simple quantitative HPLC method for measuring physiologic amino acids in cerebrospinal fluid without pretreatment, Clin Chem. 50(3), 665-669 (2004).

E. Bayer, E. Grom, B. Kaltenegger, R. Uhmann, Separation of amino acids by high performance liquid chromatography, Anal. Chem. 48(8), 1106–1109 (1976).

X, Xiao J, Huang X, Gu Z, Optimization of dansyl derivatization and chromatographic conditions in the determination of neuroactive amino acids of biological samples, Clinica Chimica Acta. 366(1-2), 352-356 (2006).

B. Reitsma, E. Yeung, Optical activity and ultraviolet absorbance detection of dansyl L-amino acids separated by gradient liquid chromatography, Anal. Chem. 59(7), 1059-1061 (1987).

I. Varzaru, A. E. Untea, T. Martura, M. Olteanu, T. D. Panaite, M. Schitea, Development and validation of an RP-HPLC method for methionine, cystine and lysine separation and determination in corn samples, Ilie.Van.Rev.Chem. 64, 673-679 (2013).

Cs. Albert, K. Lóki, É. Varga-Visi, G. Pohn, P. Sára, J. Csapó: Separation and determination of sulphur containing amino acid enantiomers by High Performance, Krmiva, 4, 187-191 (2006).

A. L. V. Villa, M. R. S. Aragão, E. P. dos Santos, A. M. Mazotto, R. B. Zingali, E. P. de Souza, , A. B.Vermelho, Feather keratin hydrolysates obtained from microbial keratinases: effect on hair fiber. BMC Biotechnology. 13(1), 15 (2013). doi:10.1186/1472-6750-13-15

Downloads

Additional Files

Published

2018-12-03

How to Cite

Sherovski, P., Stefova, M., & Ristovska, N. (2018). Simultaneous RP-HPLC-DAD determination of dansyl amino acids in chemically treated human hair. Macedonian Journal of Chemistry and Chemical Engineering, 37(2), 135–144. https://doi.org/10.20450/mjcce.2018.1594

Issue

Vol. 37 No. 2 (2018)

Section

Analytical Chemistry

License

The authors agree to the following licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

  • Adapt — remix, transform, and build upon the material
  • for any purpose, even commercially.
The licensor cannot revoke these freedoms as long as you follow the license terms.

 Under the following terms:

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

  • NonCommercial — You may not use the material for commercial purposes.

Most read articles by the same author(s)

> >>