Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method


  • Tanja Petreska Ivanovska Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 Skopje
  • Lidija Petruševska-Tozi Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 Skopje
  • Margita Dabevska Kostoska Center for Public Health – Tetovo, 29 Noemvri bb, Tetovo
  • Nikola Geškovski Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 Skopje
  • Anita Grozdanov Faculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Rudjer Bošković 16, 1000 Skopje
  • Chris Stain sity of Reading, Centre for Advanced Microscopy, Reading RG6 6AH, Whiteknights
  • Trajče Stafilov Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje
  • Kristina Mladenovska Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 Skopje




Lactobacillus casei, chitosan-Ca-alginate microparticles, spray-drying meth


In this study, the probiotic Lactobacillus casei was microencapsulated using the method of spray-drying combined with polyelectrolyte complexation of alginate, fructooligosaccharide and chitosan, and cross-linking with calcium chloride, followed by freeze-drying. Survival rate and physicochemical properties of the prepared microparticles were evaluated. In addition, viability of Lactobacillus casei in simulated gastric and intestinal juices was investigated. Positively charged microparticles with average size of 11.08±1.1 μm and high cell viability of 10.98±0.11 log cfu/g were prepared. The synbiotic microparticles were stable during exposure to simulated gastric and intestinal juices, while release of viable cells above the therapeutic value (8.31±0.14 log cfu/g) in the simulated colonic pH was observed.
The presented method for microencapsulation of synbiotics shows potential for effective protection of viable probiotic cells during exposure to harsh environmental conditions.


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How to Cite

Petreska Ivanovska, T., Petruševska-Tozi, L., Dabevska Kostoska, M., Geškovski, N., Grozdanov, A., Stain, C., Stafilov, T., & Mladenovska, K. (2012). Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method. Macedonian Journal of Chemistry and Chemical Engineering, 31(1), 115–123. https://doi.org/10.20450/mjcce.2012.64



Pharmaceutical Engineering

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