Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method

Authors

  • Tanja Petreska Ivanovska Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 Skopje
  • Lidija Petruševska-Tozi Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 Skopje
  • Margita Dabevska Kostoska Center for Public Health – Tetovo, 29 Noemvri bb, Tetovo
  • Nikola Geškovski Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 Skopje
  • Anita Grozdanov Faculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Rudjer Bošković 16, 1000 Skopje
  • Chris Stain sity of Reading, Centre for Advanced Microscopy, Reading RG6 6AH, Whiteknights
  • Trajče Stafilov Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje
  • Kristina Mladenovska Faculty of Pharmacy, Ss. Cyril and Methodius University, Vodnjanska 17, 1000 Skopje

DOI:

https://doi.org/10.20450/mjcce.2012.64

Keywords:

Lactobacillus casei, chitosan-Ca-alginate microparticles, spray-drying meth

Abstract

In this study, the probiotic Lactobacillus casei was microencapsulated using the method of spray-drying combined with polyelectrolyte complexation of alginate, fructooligosaccharide and chitosan, and cross-linking with calcium chloride, followed by freeze-drying. Survival rate and physicochemical properties of the prepared microparticles were evaluated. In addition, viability of Lactobacillus casei in simulated gastric and intestinal juices was investigated. Positively charged microparticles with average size of 11.08±1.1 μm and high cell viability of 10.98±0.11 log cfu/g were prepared. The synbiotic microparticles were stable during exposure to simulated gastric and intestinal juices, while release of viable cells above the therapeutic value (8.31±0.14 log cfu/g) in the simulated colonic pH was observed.
The presented method for microencapsulation of synbiotics shows potential for effective protection of viable probiotic cells during exposure to harsh environmental conditions.

References

T. Mattila-Sandholm, P. Myllarinen, R. Crittenden, G. Mogensen, R. Fondè, and M. Saarela, Technological challenges for future probiotic foods, Int. Dairy. J. 12, 173–183 (2002).

N. Betoret, L. Puente, M. J. Diaz, M. Pagán, M. J. Garcia, M.L. Gras, J. Martinez-Monzó and P. Fito, Development of probiotic-enriched dried fruits by vacuum impregnation, J. Food Eng. 56, 273–277 (2003).

C. Desmond, C. Stanton, G. F. Fitzgerald, K. Collins and R.P. Ross, Environmental adaptation of probiotic lactobacilli towards improvement of performance during spray drying, Int. Dairy J. 12, 183–190 (2002).

FAO/WHO. Guidelines for the evaluation of probiotics in food. Joint FAO/WHO working group report on drafting guidelines for the evaluation of probiotics in food, London, Ontario, Canada (2002), http://www.who.int/foodsafety/fs_management/ en/probiotic_guidelines.pdf

A. K. Anal and H. Singh, Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery, Trends in Food Sci. Technol. 18, 240–251 (2007).

W. Krasaekoopt, B. Bhandari and H. Deeth, The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria, Int. Dairy J. 14, 737– 743 (2004).

P. Capela, T. K. C. Hay and N. P. Shah, Effect of cryoprotectants, prebiotics and microencapsulation on survival of probiotic organisms in yogurt and freeze dried yoghurt, Food Res. Int. 39, 203– 211 (2006).

K. N. Chen, M. J. Chen and C. W. Lin, Optimal combination of the encapsulating materials for probiotic microcapsules and its experimental verification (R1), J. Food Eng. 79, 313–320 (2006).

N. T. Annan, A. D. Borza and L. Truelstrup Hansen, Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal condition, Food Res. Int. 41, 184–193 (2008).

G. K. Gbassi, T. Vandamme, S. Ennahar and E. Marchioni, Microencapsulation of Lactobacillus plantarum spp. in an alginate matrix coated with whey protein, Int. J. Food Microbiol. 129, 103– 105 (2009).

T. Heidebach, P. Först and U. Kulozik, Influence of casein-based microencapsulation on freeze-drying and storage of probiotic cell, J. Food Eng. 98, 309–316 (2010).

O. Sandoval-Castilla, C. Lobato-Calleros, H. S. Garcia-Galindo, J. Alvarez-Ramirez and E. J. Vernon-Carter, Textural properties of alginate-pectin beads and survivability of entrapped Lb. casei in simulated gastrointestinal conditions and in yoghurt, Food Res. Int. 43, 111–117 (2010).

S. Rokka and P. Rantamäki, Protecting probiotic bacteria by microencapsulation: challenges for industrial applications, Eur. Food Res. Technol. 231, 1–12 (2010).

W. Krasaekoopt, B. Bhandari and H. Deeth, Evaluation of encapsulation techniques of probiotics for yoghurt, Int. Dairy J. 13, 3–13 (2003).

M. Chávarri, A. Marañón, R. Ares, F.C. Ibáñez, F. Marzo and M. del Carmen Villarán, Microencapsulation of a probiotic and prebiotic in alginatechitosan capsules improves survival in simulated gastro-intestinal conditions, Int. J. Food Microbiol. 142, 185–189 (2010).

K. Kailasapathy, Microencapsulation of probiotic bacteria: technology and potential applications, Curr. Issues Intest. Microbiol. 3, 39–48 (2002).

A. Picot and C. Lacroix, Encapsulation of bifidobacteria in whey protein-based microcapsules and survival in simulated gastrointestinal conditions and in yoghurt, Int. Dairy J. 14, 505–515 (2004).

T. Petrovic, V. Nedovic, S. Dimitrijevic-Brankovic, B. Bugarski and C. Lacroix, Protection of probiotic microorganisms by microencapsulation, CI& CEQ, 13, 169–174 (2007).

B. M. Corcoran, R. P. Ross, G. F. Fitzgerald, and C. Stanton, Comparative survival of probiotic lactobacilli spray-dried in the presence of prebiotic substances, J. Appl. Microbiol. 96, 1024–1039 (2004).

C. A. Morgan, N. Herman, P. A. White and G. Vesey, Preservation of micro-organisms by drying; A review, J. Microbiol. Methods 66, 183–193 (2006).

N. S. A. M. Akhiar, Enhancement of probiotics survival by microencapsulation with alginate and prebiotics, Basic Biotechnol. 6, 13–18 (2010).

K. Mladenovska, R. S. Raicki, E. I. Janevik, T. Ristoski, M. J. Pavlova, Z. Kavrakovski, M. G. Dodov and K. Goracinova, Colon-specific delivery of 5-aminosalicylic acid from chitosan-Ca-alginate microparticles, Int. J. Pharm. 342, 124–136 (2007a).

K. Mladenovska, O. Cruaud, P. Richomme, E. Belamie, R. S. Raicki, M. C. Venier-Julienne, E. Popovski, J. P. Benoit and K. Goracinova, 5-ASA loaded chitosan-Ca-alginate microparticles: Preparation and physicochemical characterization, Int. J. Pharm. 345, 59–69 (2007b).

E. B. Minelli, A. Benini, A. M. Marzotto, A. Sbarbati, O. Ruzzenente, R. Ferrario, H. Hendriks and F. Dellaglio, Assessment of novel probiotic Lactobacillus casei strains for production of functional dairy foods, Int. Dairy J. 14, 723–736 (2004).

R. R. Mokarram, S. A. Mortazavi, M. B. H. Najafi and F. Shahidi, The influence of multi stage alginate coating on survivability of potential probiotic bacteria in simulated gastric and intestinal juice, Food Res. Int. 42, 1040–1045 (2009).

S. Mandal, A. K. Puniya and K. Sing, Effect of alginate concentrations on survival of microencapsulated Lactobacillus casei NCDC-298 Int. Dairy J. 16, 1190–1195 (2006).

P. Allan-Wojtas, L. Truelstrup-Hansen and A. T. Paulson, Microstructural studies of probiotic bacteria- loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation, LWT Food Sci. Technol. 41, 101–108 (2008).

W. Krasaekoopt, B. Bhandari and H. C. Deeth, Survival of probiotics encapsulated in chitosancoated alginate beads in yoghurt from UHT- and conventionally treated milk during storage, LWTFood Sci. Technol. 39, 177–183 (2006).

D. J. Pimentel-González, R. G. Campos-Montiel, C. Lobato-Calleros and R. Pedroza-Islas, Encapsulation of Lactobacillus rhamnosus in double emulsions formulated with sweet whey as emulsifier and survival in simulated gastrointestinal conditions, Food Res. Int. 42, 292–297 (2009).

D. Semyonov, O. Ramon, Z. Kaplun, L. Levin- Brener, N. Gurevich and E. Shimoni, Microencapsulation of Lactobacillus paracasei by spray freeze drying, Food Res. Int. 43, 193–202 (2010).

G. Fundueanu, C. Nastruzzi, A. Carpov, J. Desbrieres and M. Rinaudo, Physico-chemical characterization of Ca-alginate microparticles produced with different methods, Biomaterials 20, 1427– 1435 (1999).

V. Chandramouli, K. Kailasapathy, P. Peiris and M. Jones, An improved method of microencapsulation and its evaluation to protect Lactobacillus spp. in simulated gastric condition, J. Microbiol. Methods 56, 27–35 (2004).

Y. Murata, S. Toniwa, E. Miyamoto, S. Kawashima, Preparation of alginate gel beads containing chitosan salt and their function, Int. J. Pharm. 176, 265–268 (1999).

S. Koo, Y. Cho, C. Huh, Y. Baek and J. Park, Improvement of stability of Lactobacillus casei YIT9018 by microencapsulation using alginate and chitosan, J. Microbiol. Biotechnol. 11, 376–383 (2001).

S.-H. Song, Y.-H. Cho and J. Park, Microencapsulation of Lactobacillus casei YIT 9018 using a microporous glass membrane emulsification system, J. Food Sci. 68, 195–200 (2003).

P. C. Teixeira, M. H. Castro, F. X. Malcata and R. M. Kirby, Survival of Lactobacillus-delbrueckii ssp. bulgaricus following spray-drying, J. Dairy Sci. 78, 1025–1031 (1995).

L. Truelstrup Hansen, P. M. Allan-Wojtas, Y. L. Jin and A. T. Paulson, Survival of Ca-alginate microencapsulated Bifidobacterium spp. in milk and simulated gastrointestinal conditions, Food Microbiol. 19, 35–45 (2002).

A. Sohail, M. S. Turner, A. Coombes, T. Bostrom and B. Bhandari, Survivability of probiotics encapsulated in alginate gel microbeads using a novel impinging aerosols method, Int. J. Food Microbiol. 145, 162–168 (2011).

M. George and T. E. Abraham, Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan. A review, J. Control. Release 114, 1–14 (2006).

L-M. Zhao, L-E. Shi, Z-L. Zhang, J-M. Chen, D-D. Shi, J. Yang and Z. X. Tang, Preparation and application of chitosan nanoparticles and nanofibers, Braz. J. Chem. Eng. 28, 353–362 (2011).

R. Hejazi and M. Amiji, Chitosan-based gastrointestinal delivery systems, J. Control. Release 89, 151–165 (2003).

Downloads

Published

2012-06-15

How to Cite

Petreska Ivanovska, T., Petruševska-Tozi, L., Dabevska Kostoska, M., Geškovski, N., Grozdanov, A., Stain, C., Stafilov, T., & Mladenovska, K. (2012). Microencapsulation of <i>Lactobacillus casei</i> in chitosan-Ca-alginate microparticles using spray-drying method. Macedonian Journal of Chemistry and Chemical Engineering, 31(1), 115–123. https://doi.org/10.20450/mjcce.2012.64

Issue

Section

Pharmaceutical Engineering

Most read articles by the same author(s)

> >>