Optimization and validation of a derivatization method for analysis of biogenic amines in wines using RP-HPLC-DAD

Authors

  • Krste Tashev State Phytosanitary Laboratory, Ministry of Agriculture, Forestry and Water Economy, Skopje
  • Violeta Ivanova-Petropulos Faculty of Agriculture, University “Goce Delčev”, Krste Misirkov bb, Štip, Republic of Macedonia
  • Marina Stefova Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Arhimedova 5, Skopje

DOI:

https://doi.org/10.20450/mjcce.2016.884

Keywords:

wine, biogenic amines, HPLC, derivatization, dansyl chloride, validation

Abstract

An analytical method for determination of the biogenic amines: tryptamine, putrescine  histamine, phenylethylamine, tyramine, cadaverine, spermidine and spermine in red and white wines using pre-column derivatization with dansyl chloride (DnsCl) and reversed phase-high performance liquid chromatography with diode array detector (RP-HPLC-DAD) was developed and optimized. Concentration of DnsCl, pH, time of reaction and evaporation under nitrogen were tested and optimized. Best recoveries, ranging from 72 to 102 % were obtained for all analyzed amines applying derivatization with 10 mg/mL of DnsCl for 1 h at 60 oC and pH 9. The developed method showed excellent linearity with R2>0.99 for all tested amines. The accuracy was checked with the standard addition method showing satisfactory recovery, good repeatability and reproducibility. The optimized and validated method was applied for analysis of white and red wines from Macedonian origin, observing higher total amounts of biogenic amines in red wines. 

Author Biographies

Krste Tashev, State Phytosanitary Laboratory, Ministry of Agriculture, Forestry and Water Economy, Skopje

Head of Department

Violeta Ivanova-Petropulos, Faculty of Agriculture, University “Goce Delčev”, Krste Misirkov bb, Štip, Republic of Macedonia

professor

Marina Stefova, Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Arhimedova 5, Skopje

professor

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Published

2016-04-18

How to Cite

Tashev, K., Ivanova-Petropulos, V., & Stefova, M. (2016). Optimization and validation of a derivatization method for analysis of biogenic amines in wines using RP-HPLC-DAD. Macedonian Journal of Chemistry and Chemical Engineering, 35(1), 19–28. https://doi.org/10.20450/mjcce.2016.884

Issue

Vol. 35 No. 1 (2016)

Section

Analytical Chemistry

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