Determination of dimethoate, 2,4-dichlorophenoxy acetic acid, mecoprop and linuron pesticides in environmental waters in Republic of Macedonia by high performance liquid chromatography


  • Aleksandra Nestorovska-Krsteska Ministry of Environment and Physical Planning, Drezdenska 52, Skopje,
  • Meri Mirčeska Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril & Methodius University, Skopje,
  • Jean-Jacques Aaron ITODYS, Université Paris 7, CNRS UMR 7086, 1, rue Guy de la Brosse, 75005 Paris, & Université Paris–Est Marne-la-Vallée, Laboratoire Géomatériaux et Géologie de l'Ingénieur, EA 4119, 5, bld Descartes, 77454 Marne–la-Vallée Cedex 02,
  • Zoran Zdravkovski



dimethoate, 2, 4-dichlorophenoxy acetic acid, linuron, mecoprop, HPLC-UV-DAD, SPE, environmental waters


An HPLC-UVDAD method for determination of dimethoate, 2,4-dichlorophenoxy acetic acid (2,4-D), mecoprop (MCPP) and linuron in environmental waters was developed. The water samples were concentrated and extracted by a solid phase extraction (SPE) method on Bond Elut PPL cartridges. After extraction the investigated compounds were separated on Stability RP Pesticides chromatographic column using mobile phase composed of acetonitrile- water-acetic acid in volume fractions of 39:59:2 and flow rate of 0.7 mL/min. Ultraviolet absorption detection was carried out for dimethoate, 2,4-D and MCPP at 229 nm, and for linuron at 249 nm. Recoveries made from 500 mL of drinking waters using solid phase extraction ranged between 64.3–92.1 %. The method was applied to environmental waters in Macedonia that receive runoffs from agriculture lands. The levels of pesticides under study ranged between 0.31 μg/L and 7.05 μg/L, depending on the compound and sampling period.


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How to Cite

Nestorovska-Krsteska, A., Mirčeska, M., Aaron, J.-J., & Zdravkovski, Z. (2008). Determination of dimethoate, 2,4-dichlorophenoxy acetic acid, mecoprop and linuron pesticides in environmental waters in Republic of Macedonia by high performance liquid chromatography. Macedonian Journal of Chemistry and Chemical Engineering, 27(1), 25–33.



Environmental Chemistry

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