Solvolysis kinetic study and direct spectrofluorimetric analysis of the fungicide benomyl in natural waters


  • Diène Diègane Thiare University CAD, Dakar
  • Abdourakhmane Khonté University CAD, Dakar
  • Diegane Sarr University CAD, Dakar
  • Cheikh Diop University CAD, Dakar
  • Mame Diabou Gaye-Seye University CAD, Dakar
  • Atanasse Coly University CAD, Dakar
  • François Delattre Université du Littoral Côte d'Opale
  • Alphonse Tine University CAD, Dakar
  • Jean-Jacques Aaron Paris-Est Marne-la-Vallee University, Paris



benomyl, solvolysis kinetics, solvent effects, direct fluorescence analytical method, water analysis.


A direct spectrofluorimetric method for the quantitative analysis of benomyl in natural waters is described. Benomyl is an instable, fluorescent fungicide that mainly decomposes into carbendazim and n-butyl-isocyanate in organic and aqueous solutions. The kinetics of benomyl solvolysis reactions were investigated in organic solvents (methanol and acetonitrile) and in aqueous solvent systems, including β–cyclodextrin (β-CD), sodium dodecyl sulfate (SDS), dodecyltrimethylammonium chloride (DTAC), cetyltrimethylammonium chloride (CTAC), cetyltrimethylammonium hydroxide (CTAOH), Brij-700, Triton X-100 and water, at different pH and/or NaOH concentrations. The benomyl fluorescence signal was found to be quasi-completely stable in 10-2 M NaOH aqueous solution, various alkaline (10-2 M NaOH) organized media, β-CD neutral solution and Triton X-100 aqueous solutions of different pH. Based on these results, a direct spectrofluorimetric analytical method was developed for the determination of benomyl in 10-2 M NaOH aqueous solution and Triton X-100 solutions (pH7 and 10-2 M NaOH), with wide linear dynamic range (LDR) values of two to three orders of magnitude, very low limit of detection (LOD) and limit of quantification (LOQ) values of, respectively, 0.002-0.5 ng/mL and 0.007-2.0 ng/mL, and small relative standard deviation (RSD) values of 0.2-1.7 %, according to the medium. This direct spectrofluorimetric method was applied to the evaluation of benomyl residues in natural waters, with satisfactory recovery values (87-94%).

Author Biography

Jean-Jacques Aaron, Paris-Est Marne-la-Vallee University, Paris

Laboratory Geomateriaux and Environment

Full Professor (Emeritus)


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How to Cite

Thiare, D. D., Khonté, A., Sarr, D., Diop, C., Gaye-Seye, M. D., Coly, A., Delattre, F., Tine, A., & Aaron, J.-J. (2014). Solvolysis kinetic study and direct spectrofluorimetric analysis of the fungicide benomyl in natural waters. Macedonian Journal of Chemistry and Chemical Engineering, 33(2), 237–248.



Environmental Chemistry

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