Spectrofluorimetric determination of beta-blockers atenolol and bisoprolol fumarate residues in Senegal natural waters


  • Coumba Gueye University of Dakar, Senegal
  • Pape Abdoulaye Diaw University C.A. Diop of Dakar, Senegal
  • Moussa Mbaye University C.A. Diop of Dakar, Senegal
  • Olivier Maurice Aly Mbaye University C.A. Diop of Dakar, Senegal
  • Lamine Cissé University C.A. Diop of Dakar, Senegal
  • Diabou Gaye Seye University C.A. Diop of Dakar, Senegal
  • Jean-Jacques Aaron Paris-Est Marne-la-Vallee University, Paris
  • Nihal Oturan Université Paris-Est, Marne la Vallée, France
  • Mehmet Oturan Université Paris-Est, Marne la Vallée, France




Atenolol; spectrofluorimetry; water analysis


A spectrofluorimetric method was developed to determine residues of two β-blockers, atenolol (AT) and bisoprolol fumarate (BF), in Senegal's natural waters. The electronic absorption and fluorescence spectral properties of both β-blockers were investigated in several organic solvent mixtures [e.g., MeOH/H2O (60/40 v/v), cyclodextrins (β-cyclodextrin, HP-β-CD], and in the presence of surfactants (SDS, Triton X, Tween 80). After optimization, satisfactory analytical figures of merit were obtained for the determination of both β-blockers: concentration linear dynamic range of over one to two orders of magnitude, limits of detection (LODs) from 1.3 to 5.4 ng/ml for BF and from 1.2 to 3.7 ng/ml for AT, limits of quantification (LOQs) from 4.5 to 18.1 ng/ml for BF and from 4.0 to 12.5 ng/ml for AT. Relative standard deviations (RSDs) were between 2.1 and 5.3 %, according to the β-blockers. The spectrofluorimetric method was applied to the analysis of fortified river water and wastewater (effluent) collected in Senegal and France and spiked with both β-blockers. It yielded good recovery values, from 93.3 to 107.8 % for AT and from 97.4 to 108.9 % for BF. Our results demonstrated the simplicity, rapidity, and sensitivity of the spectrofluorimetric method to quantify residues of β-blockers in environmental waters.

Author Biographies

Coumba Gueye, University of Dakar, Senegal

Department of Chemistry, graduate student

Pape Abdoulaye Diaw, University C.A. Diop of Dakar, Senegal

Department of Chemistry


Moussa Mbaye, University C.A. Diop of Dakar, Senegal

Department of Chemistry


Olivier Maurice Aly Mbaye, University C.A. Diop of Dakar, Senegal

Department of Chemistry


Lamine Cissé, University C.A. Diop of Dakar, Senegal

Department of Chemistry

Associate Professor

Diabou Gaye Seye, University C.A. Diop of Dakar, Senegal

Departmentof Chzmistry



Jean-Jacques Aaron, Paris-Est Marne-la-Vallee University, Paris

Laboratory Geomateriaux and Environment

Full Professor (Emeritus)

Nihal Oturan, Université Paris-Est, Marne la Vallée, France

Laboratoire Géomatériaux Environnement (LGE)


Mehmet Oturan, Université Paris-Est, Marne la Vallée, France

Laboratoire Géomatériaux Environnement (LGE)



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Additional Files


2023-06-27 — Updated on 2023-07-01


How to Cite

Gueye, C., Diaw, P. A., Mbaye, M., Mbaye, O. M. A., Cissé, L. ., Gaye Seye, D., Aaron, J.-J., Oturan, N., & Oturan, M. (2023). Spectrofluorimetric determination of beta-blockers atenolol and bisoprolol fumarate residues in Senegal natural waters. Macedonian Journal of Chemistry and Chemical Engineering, 42(1), 79–92. https://doi.org/10.20450/mjcce.2023.2680 (Original work published June 27, 2023)



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