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Characterization of tea water extracts and their utilization for dyeing and functionalization of fabrics of different chemical compositions


  • Leposava Pavun University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia
  • Danijela Stojanović University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia
  • Aleksandra Ivanovska University of Belgrade, Innovation Center of the Faculty of Technology and Metallurgy
  • Jelena Lađarević University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia
  • Marina Milenković University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia
  • Snežana Uskoković-Marković University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia



Tea water extract, Fabric, Functionalization, Dyeing, Antioxidant and antimicrobial activity


Green, black, rooibos, and hibiscus tea (GT, BT, RT, and HT) aqueous extracts were prepared and characterized in terms of total flavonoids (TFC) and total phenolic (TPC) contents and antioxidant and antimicrobial activities. BT has the highest, while HT has the lowest TFC (1213 vs. 415 mg l–1), while the extracts’ TPCs (2283 – 7251 mg l–1) decreased in the following order: BT > GT > RT > HT. Their antioxidant activities of 78.1 – 93.1% and 97.8 – 100% were determined according to DPPH and ABTS methods, respectively. BT and especially GT aqueous extracts possessed mild effects against several microorganisms. All examined extracts have an affinity for dyeing wool, cellulose acetate, polyamide, and cotton, which is proven by the color strength values of 1.65–19.12. Wool, polyacrylonitrile, polyester, polyamide, cotton, and cellulose acetate functionalized with GT aqueous extract inhibited the growth of S. aureus and E. coli, while polyacrylonitrile and cotton also inhibited the growth of E. faecalis, and C. albicans, respectively. Wide inhibition zones for S. aureus were observed for fabrics functionalized with BT aqueous extract. Generally, the investigated fabrics showed very high (81.60 – 100%) ABTS radical scavenging ability independent of the extract used. TPCs have good linear correlations with the antioxidant activities of wool and polyacrylonitrile determined by the DPPH method. Fabrics with different chemical compositions dyed and/or functionalized with GT or BT aqueous extracts can be used to produce high-value-added medical textiles with therapeutic, prophylactic, and protective functions. They can find potential applications in wound treatment, especially in skin wounds that are susceptible to infection with S. aureus. Moreover, wool and cotton functionalized with GT or BT aqueous extract can also be considered for use in disposable medical textiles like bandages and gauze used in the wound-healing process.


(1) Textile Dyes Pollution: The Truth About Fashion’s Toxic Colours. (accessed 2022 - 12 -27)

(2) Dyeing for fashion: Why the clothes industry is causing 20% of water pollution. (accessed 2022 - 12 -27)

(3) Haji, A.; Rahimi, M. RSM Optimization of Wool Dyeing with Berberis Thunbergii DC Leaves as a New Source of Natural Dye. J. Nat. Fibers., 2022, 19 (8), 2785–2798.

(4) Mansour, R.; Dhouib, S.; Sakli, F., UV Protection and dyeing properties of wool fabrics dyed with aqueous ex-tracts of madder roots, chamomiles, pomegranate peels, and apple tree branches barks. J. Nat. Fibers. 2022, 19 (2), 610–620.

(5) Joshi, S.; Kambo, N.; Dubey, S.; Shukla, P.; Pandey, R. Effect of onion (Allium cepa L.) peel extract-based nanoemulsion on anti-microbial and UPF properties of cotton and cotton blended fabrics. J. Nat. Fibers. 2022, 19 (14), 8345–8354.

(6) Ivanovska, A.; Savić Gajić, I.; Lađarević, J.; Milošević, M.; Savić, I.; Mihajlovski, K.; Kostić, M. Sustainable dyeing and functionalization of different fibers using or-ange peel extract’s antioxidants. Antioxidants 2022, 11 (10), 2059.

(7) Ivanovska, A.; Veljović, S.; Reljić, M.; Lađarević, J.; Pavun, L.; Natić, M.; Kostić, M., Closing the Loop: Dye-ing and Adsorption Potential of Mulberry Wood Waste. J. Nat. Fibers. 2022, 19 (15), 11050–11063.

(8) Hernández, V. A.; Galleguillos, F.; Thibaut, R.; Müller, A., Fungal dyes for textile applications: testing of indus-trial conditions for wool fabrics dyeing. J. Text. I. 2019, 110 (1), 61–66.

(9) Janković, V.; Marković, D.; Nikodinović-Runić, J.; Ra-detić, M.; Ilić-Tomić, T., Eco-friendly dyeing of polyam-ide and polyamide-elastane knits with living bacterial cul-tures of two Streptomyces sp. Strains. World. J. Microbi-ol. Biot. 2023, 39, 32.

(10) Dying for colour: Toxic dyes in the textile industry (accessed 2023 - 01 - 10)

(11) Kumari, A.; Kumar, D., Evaluation of antioxidant and cytotoxic activity of herbal teas from Western Himalayan region: a comparison with green tea (Camellia sinensis) and black tea. Chem. Biol. Technol. Ag. 2022, 9, 33.

(12) Čuk, N.; Šala, M.; Gorjanc, M., Development of antibacterial and UV protective cotton fabrics using plant food waste and alien invasive plant extracts as reducing agents for the in-situ synthesis of silver nanoparticles. Cellulose 2021, 28, 3215–3233.

(13) Pavun, L.; Đurđević, P.; Jelikić-Stankov, M.; Đikanović, D.; Uskoković-Marković, S., Determination of flavo-noids and total polyphenol contents in commercial apple juices. Czech. J. Food Sci. 2018, 36 (3), 233–238.

(14) Gardner, P. T.; White, T. A. C.; McPhail, D. B.; Duthie, G. G., The relative contributions of vitamin C, carote-noids and phenolics to the antioxidant potential of fruit juices. Food. Chem. 2000, 68 (4) 471–474.

(15) Lađarević, J.; Božić, B.; Matović, L.; Božić Nedelj¬ković, B.; Mijin, D., Role of the bifurcated intramolecular hy-drogen bond on the physico-chemical profile of the novel azo pyridone dyes. Dyes Pigments 2019, 162, 562–572.

(16) CLSI M07: Methods for Dilution Antimicrobial Suscepti-bility Tests for Bacteria That Grow Aerobically (2018).

(17) Yang, R.; He, C.; Pan, B.; Wang, Z., Color-matching model of digital rotor spinning viscose mélange yarn based on the Kubelka–Munk theory. Text. Res. J. 2022, 92 (3-4), 574–584.

(18) Glaser, T. K.; Plohl, O.; Vesel, A.; Ajdnik, U.; Ulrih, N. P.; Hrnčič, M. K.; Bren, U.; Fras Zemljič, L., Functionalization of polyethylene (PE) and polypropylene (PP) material using chitosan nanoparticles with incorporated resveratrol as potential active packaging. Materials 2019, 12 (13), 2118.

(19) Hong, К. H., Polym. Bull. (2022) (Article in press)

(20) CLSI M02: Performance Standards for Antimicrobial Disk Susceptibility Tests (2018).

(21) Gresham, B. J., Antimicrobials for synthetic fibers. In: Bioactive Fibers and Polymers; American Chemical Society, Washington, 2001.

(22) Kaur, A.; Suri, R.; Rana, K.; Thakur, V.; Preeti; Dimri, P.; Mittal, N., Antioxidant levels in Indian rose, hibiscus, chrysanthemum and marigold tea and their comparison with black and green tea. Int. Res. J. Pharm. 2019, 10 (10), 52–55.

(23) Aboagye, G.; Tuah, B.; Bansah, E.; Tettey, C.; Hunkpe, G., Comparative evaluation of antioxidant properties of lemongrass and other tea brands. Sci. Afr. 2021, 11, e00718.

(24) Büyükbalci, A.; El, S. N., Determination of In Vitro anti-diabetic effects, antioxidant activities and phenol contents of some herbal teas. Plant. Foods Hum. Nutr. 2008, 63, 27–33.

(25) Akhter, K.; Ghous, T.; Ul abdin, Z.; Sadaf, E.; Hassan, A.; Irshad, A.; Andleeb, S., Pharmacological approach to glycemic treatment using black, green and herbal tea ex-tracts. Bangl. J. Bot. 2021, 50 (3), 491–498.

(26) Samadi, S.; Fard, F. R., Phytochemical properties, antiox-idant activity and mineral content (Fe, Zn and Cu) in Ira-nian produced black tea, green tea and roselle calyces. Bi-ocatal. Agr. Biotechnol. 2020, 23, 101472.

(27) Oh, J.; Jo, H.; Cho, A. R.; Kim, S.-J.; Han, J., Antioxi-dant and antimicrobial activities of various leafy herbal teas. Food Control 2013, 31 (2), 403–409.

(28) Paiva, L.; Rego, C.; Lima, E.; Marcone, M.; Baptista, J., Comparative analysis of the polyphenols, caffeine, and antioxidant activities of green tea, white tea, and flowers from Azorean Camellia sinensis varieties affected by dif-ferent harvested and processing conditions. Antioxidants 2021, 10 (2), 183.

(29) Nadiah, N. I.; Cheng, L. H.; Azhar, M. E.; Karim, A. A.; Uthumporn, U.; Ruri, A. S., Determination of phenolics and antioxidant properties in tea and the effects of poly-phenols on alpha-amylase activity. Pak. J. Nutr. 2015, 14 (11), 808–817.

(30) Vural, N.; Algan Cavuldak, Ö.; Akay, M. A.; Ertan Anlı R., Determination of the various extraction solvent effects on polyphenolic profile and antioxidant activities of se-lected tea samples by chemometric approach. J. Food Meas. Charact. 2020, 14, 1286–1305.

(31) Platzer, M.; Kiese, S.; Tybussek, T.; Herfellner, T.; Schneider, F.; Schweiggert-Weisz, U.; Eisner, P., Radical scavenging mechanisms of phenolic compounds: a quan-titative structure-property relationship (QSPR) study. Front. Nutr. 2022, 9, 882458.

(32) Kramar, A.; Petrović, M.; Mihajlovski, K.; Mandić, B.; Vuković, G.; Blagojević, S.; Kostić, M., Selected aromatic plants extracts as an antimicrobial and antioxidant finish for cellulose fabric-direct impregnation method. Fibers. Polym. 2021, 22 (12), 3317–3325.

(33) Lencova, S.; Stiborova, H.; Munzarova, M.; Demnerova, K.; Zdenkova, K., Potential of polyamide nanofibers with natamycin, rosemary extract, and green tea extract in ac-tive food packaging development: interactions with food pathogens and assessment of microbial risks elimination. Front. Microbiol. 2022, 13, 857423.


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2023-11-29 — Updated on 2023-11-30


How to Cite

Pavun, L., Stojanović, D., Ivanovska, A., Lađarević, J. ., Milenković, M., & Uskoković-Marković, S. (2023). Characterization of tea water extracts and their utilization for dyeing and functionalization of fabrics of different chemical compositions. Macedonian Journal of Chemistry and Chemical Engineering, 42(2). (Original work published November 29, 2023)



Materials Chemistry

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